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期 刊 ：中国中药杂志 2007年 32卷 11期 页码：1012.

关键词：人参；西洋参；STS标记；

Keywords:Panax ginseng, Panax quinquefolius, sequence-tagged site,

摘 要 ：目的：寻找人参、西洋参的分子鉴定新方法。方法：在人参、西洋参已知rDNA序列上设计单引物进行PCR扩增,对多态性条带克隆测序,根据序列比对情况设计人参、西洋参特异引物,通过对PCR条件的优化得到人参、西洋参的STS 标记。结果：引物Pg-q36F能得到人参、西洋参的多态性条带,特异引物Pg-6F,Pg-479R仅对人参扩增474 bp条带,特异引物Pq-442F,Pq-658R仅对西洋参扩增217 bp条带,能成功鉴定人参和西洋参。结论：建立了一种获取人参、西洋参STS标记的新方法。本方法大大加快STS标记的建立过程,可为其他中药材分子标记的获得提供指导。

Abstract:Objective: Searching a new molecular method to authenticate Panax ginseng and P. quenquefolium. Method: Single primers based on rDNA sequences of Panax species were designed to obtain polymorphic bands of P. ginseng and P. quinquefolius and then sequenced. Four PCR primers (two forword and two reverse primers) specific to P. ginseng and P. quinquefolius were designed. Result: Primer Pg-6F, Pg-479R only amplified 474 bp band for P. ginseng and primer Pq-442F, Pq-658R only amplified 217 bp band for P. quinquefolius. It is indicated that the four primers could serve as specific STS primers for Panax species. Conclusion: A new way to obtain STS primers of Panax species was established. This method is more quick and efficient than SCAR-PCR method and can serve as a model to obtain molecular markers for other Chinese material medica.

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