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期 刊 ：中国中药杂志 2008年 33卷 09期 页码：1060.

关键词：沙棘总黄酮；心肌缺血；蛋白质表达；

Keywords:total flavones of Hippophae rhamnoides, myocardial ischemia, protein expression,

摘 要 ：目的:探讨沙棘总黄酮对缺血心肌的保护作用及其相关蛋白表达谱的改变。方法:应用表面增强激光解吸离子化(surface enhanced laser desorption/lonization,SELDI)蛋白质芯片技术检测了经沙棘总黄酮处理后的缺血心肌组织和未经沙棘总黄酮处理的对照组缺血心肌组织中的蛋白质谱。用PHS-C型蛋白芯片阅读机读取数据并进行比较分析。结果:与对照组相比,在经沙棘总黄酮处理后的缺血心肌组织共捕获6个存在有差异的蛋白质峰,其中有4个出现在IMAC3芯片上,1个出现在SAX2芯片上,1个出现在NP20芯片上。6个差异蛋白质峰中,有1个呈高表达,5个呈低表达。结论:沙棘总黄酮在缺血心肌组织中可调节相关蛋白表达谱从而产生保护作用。

Abstract:Objective: To study the mechanisms of the anti-myocardial ischemia of total flavones of Hippophae rhamnoides(TFH) at the level of proteome.Method: Surface enhanced laser desorption/ionization(SELDI) mass spectrometry with protein chip IMAC3,SAX2 and NP20 was performed to compare the differentially expressed protein in myocardial ischemia in the TFH-treated groups with the 0.9% sodium chloride groups.Protein chips were examined in PBSⅡ-C protein chip reader(ciphergen ciosystem inc) and the protein profiling was analyzed by Proteinchip Software 3.0.2.Result: The revealed six peaks had significant difference between the TFH-treated groups and the control groups,one of which were up-regulated in the TFH-treated groups,and the other were down-regulated.And in these six distinct proteins,there were four proteins on the IMAC3 chips and one protein on the SAX2 chips.Conclusion: The TFH could prevent the myocardium from ischemia via regulating expression of different proteins.

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