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期 刊 ：中国中药杂志 2007年 32卷 08期 页码：725.

关键词：莪术；三氧化二砷；晶状体上皮细胞；增殖；信号转导；

Keywords:Rhizoma Curcumae, arsenite trioxide, lens epithelial cell, proliferation, signal transduction,

摘 要 ：目的：探讨莪术(RC)、三氧化二砷(As₂O₃)对晶状体上皮细胞(LEC)增殖及细胞信号转导的影响,为防治后发性白内障提供实验依据。方法：采用重组人碱性成纤维细胞生长因子(rhbFGF)诱导牛LEC增殖；四甲基偶氮唑蓝法(MTT)检测RC,As₂O₃对LEC增殖的抑制率；流式细胞术(FCM)检测RC,As₂O₃对LEC增殖细胞核抗原(PCNA)蛋白表达的抑制作用；荧光分光光度法检测LEC内［Ca²⁺］i、放射免疫分析法检测cAMP和cGMP。结果：RC,As₂O₃对rhbFGF诱导的LEC增殖的抑制率增加(P＜0.01)；明显下调rhbFGF诱导增殖的LEC内PCNA蛋白表达(P＜0.01)；使增殖的LEC内［Ca²⁺］i和cAMP显著增高(P＜0.01),cGMP显著降低(P＜0.01)。上述指标均呈剂量-效应关系。结论：RC,As₂O₃能显著抑制LEC增殖；Ca²⁺,cAMP和cGMP细胞信号转导是其抑制LEC增殖的重要分子机制；RC,As₂O₃对防治后发性白内障具有广阔的前景。

Abstract:Objective: To investigate the effect of Rhizoma Curcumae (RC), arsenite trioxide (As₂O₃) on proliferation ana signal transduction molecule in lens epithelial cell (LEC), in order to provide experiment evidence for prevention and treatment of after cataract. Method: Proliferation of cultured bovine LEC were induced by induced by recombinant human basic fibroblast growth factor (rhbFGF); Inhibitory rates of LEC proliferation induced by RC, As₂O₃ were detected by methyl thiazolyl tetrazolium (MTT); Inhibitory effects of expression of proliferating cell nuclear antigen (PCNA) induced by RC, As₂O₃ in LEC were assayed via flow cytometer (FCM); Concentrations of LEC calcium (［Ca²⁺］i) were determined by spectrofluoremeter, intracellular concentrations of cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) of LEC were measured by radioimmunoassay. Result: Inhibitory rates of RC, As₂O₃ on LEC proliferation induced by rhbFGF increased significantly, showing dose-dependent (P＜0.01). PCNA expression of LEC proliferation induced by rhbFGF were down regulated obviously by RC, As₂O₃, showing dose-dependent (P＜0.01). Concentrations of ［Ca²⁺］i and cAMP increased and cGMP decreased significantly in LEC of proliferation inhibited by RC, As2O3 (P＜0.01). Conclusion: RC, As₂O₃ can inhibit LEC proliferation obviously. Signal transductions of ［Ca²⁺］i, cAMP, cGMP may be the important molecular mechanism. There are broad prospect for RC, As₂O₃ on prevention and treatment of after cataract.

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