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期 刊 ：中国中药杂志 2008年 33卷 16期 页码：2045.

关键词：川楝子；肝毒性；大鼠；机制；

Keywords:fructus toosendan, hepatotoxicity, rat, mechanism,

摘 要 ：目的：研究川楝子对大鼠SOD，MDA，γ-GT，GSH-Px及炎症因子TNF-α，NF-κB，ICAM-1等的影响，探求川楝子致大鼠肝脏损害的毒性机制。方法：SD大鼠，连续给药45 d后，取正常组及川楝子(120 g·kg^-1组)大鼠肝组织，制成10%肝匀浆，按试剂盒方法测定肝组织中超氧化物歧化酶SOD，MDA活性和γ-GT，GSH-Px，蛋白含量，并根据测得SOD与MDA计算SOD/MDA；采用双抗体夹心ABC-ELISA法测定大鼠肝组织TNF-α含量；采用免疫组织化学SABC法染色测定大鼠肝组织NF-κB p65，ICAM-1蛋白表达量。结果：与正常组比较，口服川楝子120 g·kg^-1 45 d后，大鼠肝组织SOD显著下降(P<0.01)，MDA显著上升(P<0.05)，SOD/MDA显著下降(P<0.01)；γ-GT值显著上升(P<0.01)，GSH-Px显著下降(P<0.01)；TNF-α含量升高(P<0.05)；NF-κB p65，ICAM-1阳性表达明显增强(P<0.01)。结论：大鼠灌胃给予川楝子后，可对肝脏产生明显的毒性，其机制可能与自由基及炎症因子有关。

Abstract:Objective: To study the effects on SOD, MDA, γ-GT, GSH-Px and inflammatory factor (TNF-α, NF-κB, ICAM-1) in rats that induced by fructus toosendan, and to search for the hepatotoxicity mechanism of rats that induced by fructus toosendan. Method: The SD rats were given fructus toosendan 120 g·kg^-1 by orally for 45 days, then take the liver tissue of control and fructus toosendan group to prepare liver homogenate. The activities of SOD, the content of MDA, the ratio of SOD and MDA, the content of γ-GT and glutathione peroxidase (GSH-Px) were detected according to the methods of kit. The tumor necosis factor-α (TNF-α) was detected by ABC-ELISA. The expression of NF-κB p65 and ICAM-1 were detected by immunohistochemistry. Result: The rats were given fructus toosendan 120 g·kg^-1 by orally for 45days, the SOD and GSH-Px activities in liver tissue decreased, the content of MDA increased, the ratio of SOD and MDA decreased, the content of γ-GT and TNF-α, the masculine expression of NF-κB p65 and ICAM-1 increased. Conclusion: After the rats were given fructus toosendan, the liver can be damaged obviously, and the mechanism of hepatotoxicity perhaps related to free radical and inflammatory factor.

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