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期 刊 ：中国中药杂志 2008年 33卷 16期 页码：1986.

关键词：夜香牛；新木脂素类；甾醇类；4-sulfo-benzocyclobutene；

Keywords:Vernonia cinerea, lignan, steroids, new natural product, induce NGF activity,

摘 要 ：目的：研究广西产夜香牛生物活性化学成分。方法：用鼠性肾上腺髓质嗜铬细胞瘤PC-12细胞活性跟踪筛选，柱色谱方法分离化合物，谱学方法鉴定化合物结构。结果：分离得到4个化合物，根据谱学数据分析鉴定其结构分别为木脂素类化合物(+)-lirioresinol树脂醇B(1)，甾醇类化合物豆甾醇(2)和豆甾醇3-O-β-D-葡萄糖苷(3)，以及4-sulfo-benzocyclobutene(4)；用神经细胞体外模型试验，检测了分离得到化合物的NGF诱导活性。结论：化合物1，3,4为首次从该属植物中分离得到，化合物4为新的天然产物。

Abstract:Objective: To study the constituents of the whole herbs of Vernonia cinerea. by bio-activity guided isolation with PC-12 model. Method: The constituents were separated by column chromatography and the structures were elucidated by spectroscopic methods. Result: Four compounds were identified to be (+)-lirioresinol B (1), stigmasterol (2), stigmasterol-3-O-β-D-glucoside (3), 4-sulfo-benzocyclobutene (4), and their NGF inducing activity were also investigated. Conclusion: Compounds 1, 3, 4 were isolated from this genus for the first time, and compound 4 was identified as a new natural product. Compounds 1, 3, 4 showed cytotoxicity on PC-12, and compounds 2, 3, 4 showed inhibition activity. Compound 4 showed a specific effect on the survival of TrkA fibroblasts, and resulted in the inducing NGF activity.

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