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期 刊 ：中国中药杂志 2007年 32卷 03期 页码：238.

关键词：壁虎；抗肿瘤作用；急性毒性实验；

Keywords:Gecko swinhonis, anti-tumor activity, acute toxicity testing,

摘 要 ：目的:研究干、鲜壁虎冻干粉对移植性小鼠S180肉瘤的抑制作用及干、鲜壁虎冻干粉的安全性。方法:建立移植瘤小鼠S180肉瘤模型。将80只小鼠随机分为模型组,西药组,干、鲜壁虎低、中、高组。用不同浓度的干、鲜壁虎冻干粉灌服荷瘤小鼠10 d后,分别计算抑瘤率、胸腺指数和脾脏指数。急性毒性实验:检测干、鲜壁虎半数致死剂量(LD50)及干、鲜壁虎的最大耐受剂量(LD0)。结果:干、鲜壁虎低、中、高3个剂量组的抑瘤率分别为31.4%,50.8%,37.7%和14.8%,19.1%,54.7%,且均不同程度地提高了荷瘤小鼠的胸腺重量和胸腺指数。8组小鼠的脾脏重量及脾脏指数没有明显差别(P>0.05)。急性毒性实验无法测出干、鲜壁虎冻干粉的LD50,LD0实验结果显示二者在给药量均超过人临床用量的2 000倍以上时,仅鲜壁虎组小鼠体重在灌胃后3天较对照组和干壁虎组明显减轻(P<0.01),其第5,7天体重与另两组比较均无显著差异。干壁虎组小鼠未发现体重减轻及其他异常反应。结论:干、鲜壁虎均可抑制小鼠S180肉瘤的生长,且抑瘤作用相近,其机制可能与提高机体的细胞免疫功能有关。急性毒性实验表明干、鲜壁虎常规临床用量安全可靠。

Abstract:Objective: To investigate the anti-tumor activity of dry Gekko swinhonis freeze-dried powder(DGFP) and fresh G.swinhonis freeze-dried powder(FGFP) on mice sarcoma S180 and acute toxicity testing of the two powders.Method: Mice xenotransplant model of sarcoma S180 was established.Eighty mice were randomly divided into 8 groups.Control group were orally administrated by saline,another intraperitoneally injected with 5-Fu,the other six groups were orally administrated by DGFP and FGFP,each at three different doses(low,moderate and high).Rate of restraining tumor,index of thymus and spleen were calculated after 10 days‘ treatment.Acute toxicity testing tried to figure out LD₅₀ and LD₀ of DGFP and FGFP.Result: The restraining tumor rates of DGFP and FGFP each at three doses were 31.4%,50.8%,37.7% and 14.8%,19.1%,54.7%. DGFP and FGFP elevated the thymic weight and thymic index of the mice to different extent.There were no significant differences among the eight groups in their spleen weight and spleen index.Acute toxicity testing did not figure out LD₅₀of DGFP and FGFP.In LD₀test,the administrating dosages of DGFP and FGFP given to the mice were both more than 2000 times than those given to patients on clinic.The result showed nothing abnormal in DGFP group.Compared with the DGFP and control group there was only a significant body weight decrease(P<0.01) in the FGFP group in the first three days.However,on the fifth day and the seventh day there was no significant difference.Conclusion: DGFP and FGFP have conspicuous anti-tumor effects in vivo.The mechanism may be related to the elevated cellular immune function.Acute toxicity testing reveals that DGFP and FGFP are quite safe for conventional oral use on clinic.

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