---

期 刊 ：中国中药杂志 2006年 31卷 08期 页码：646.

关键词：生首乌；二苯乙烯苷；大孔树脂；正交试验；纯化；

Keywords:Polygonum multiflori, 2, 3, 5, 4′-tetrahydroxy-stilbene-2-O-β-D-glycoside, marcoporous resin, orthogonal design, purification,

摘 要 ：目的:研究大孔吸附树脂纯化生首乌二苯乙烯苷的工艺及参数。方法:以树脂的比吸附量为指标,比较了4种大孔树脂对二苯乙烯苷的吸附性能,对大孔树脂吸附纯化生首乌中二苯乙烯苷的工艺参数进行筛选,并在此基础上考察了洗脱条件。结果:S-8树脂对二苯乙烯苷的吸附性能最好,树脂的处理量为1.6 g生药.g^-1湿树脂,上样液含量为0.2 g生药.mL^-1,流速1.5 mL.min^-1,pH 7～8,10BV(即15 mL.g^-1树脂)50%乙醇为洗脱溶剂。通过本工艺生首乌的二苯乙烯苷比吸附量达36.89 mg.g-1。结论:工艺简单,树脂再生容易,该纯化方法可取。

Abstract:Objective: To study the conditions and parameters of purifying 2,3,5,4′-tetrahydroxy-stilbene-2-O-β-D-glycoside from Polygonum multiflori.Method:Absorption capacity of four resins for 2,3,5,4′-tetrahydroxy-stilbene-2-O-β-D-glycoside was compared.With the adsorption ability as indexes, the process of absorbing and purifying 2,3,5,4′-tetrahydroxy-stilbene-2O-β-D-glycoside from P.multiflori with S-8 macroporous resin absorbent was selected by orthogonal design.Result: The S-8 resin was the best of the four resins.The optimum process condition was 50% ethanol as eluting solvent,the flow rate at 1.5 mL·min^-1,pH at 7-8,and the solution concentration at 0.2 g·mL^-1.The absorption capacity by this process was 36.89 mg·g^-1.Conclusion: The process is simple and convenient and the regeneration of resin is easy,So this method of purification is advisable.

---