Abstract:Objective: To study the chemical constituents from herb of Rhodobryum roseum. Method: The compounds were isolated by column chromatography, and identified by IR, NMR data. Result: 8 compounds were isokted and identified. They are piperine (1) , caf-feic acid methyl ester (2), uracil glucoside (3), ursolic acid (4), 5α, 8α-epidioxy-methylcholesta-6,22-dien-3β-ol (5), 5α, 8α-epidioxy-methylcholesta-6,9(11), 22-trien-3β-ol (6), β-sitosterol (7), daucosterol (8). Conclusion: 7 compounds (1-6,8) were isolated from this plant for the first time.