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期 刊 ：中国中药杂志 2005年 30卷 13期 页码：990.

关键词：哈蟆油；十六烷酸胆甾醇酯；HPLC；含量；

Keywords:Oviductus Ranae, cholesteryl palmitate, content determination, HPLC,

摘 要 ：目的:建立测定哈蟆油中的十六烷酸胆甾醇酯的含量方法。方法:采用ZorbaxSilica色谱柱,流动相为环己烷-乙醚(40:1);检测波长203nm;柱温40℃;流速1mL·min^-1。结果:十六烷酸胆甾醇酯在0.60-8.92μg具有良好线性关系,平均回收率为98.4%,RSD1.8%,具较好的精密度及稳定性。结论:HPLC测定哈蟆油中的十六烷酸胆甾醇酯的含量,不仅操作简单、准确,而且重复性好。

Abstract:Objective: To develop a new method for the determination of cholesteryl palmitate in Oviductus Ranae. Method: A HPLC method was set up, using Zorbax Silica column and cyclohexane-diethyl ether (40:1) as mobile phase with a flow rate of 1.0 mL·min^-1, and the UV detection wavelength was 203 nm. Result: The calibration curve was linear over the range of 0.60-8.92 μg(r= 0.999 7) , the average recovery of the method was 98.4% . RSD 1.8% (n= 6) . Conclusion: The results showed that method was reliable and accurate.

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