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期 刊 ：中国中药杂志 2005年 30卷 21期 页码：1685.

关键词：五倍子；洗必泰；抗菌作用；连续培养系统；生物膜模型；

Keywords:Galla Chinensis, chlorhexidine, antibacterial effect, chemostat, biofilm model,

摘 要 ：目的:通过恒化器生物膜模型比较五倍子和洗必泰的抗菌效果,为中药替代化学药物预防龋病的应用推广提供理论依据。方法:建立恒化器牙菌斑生物膜模型,经五倍子和洗必泰处理后,通过生物膜上细菌计数、连续pH的监测、生物膜超微结构观察,比较二者对口腔常见细菌的生长、产酸和生物膜表面形态的影响。结果:①五倍子和洗必泰对实验菌株的生长都有不同程度的抑制,五倍子对生物膜细菌的生态组成无明显影响。②二者都能够抑制实验细菌产酸,使恒流培养室内的终末pH高于临界pH;4 g.L^-1五倍子抑制产酸的能力同0.5 g.L^-1洗必泰相近。③与对照组相比,五倍子和洗必泰处理后的生物膜表面菌细胞界限清晰、绒团样胞外基质相对减少,二者间未见明显差别。结论:在生物膜模型中,五倍子可以通过抑制细菌生长、产酸、产胞外基质等途径,抑制生物膜细菌的生长代谢,4 g.L^-1五倍子的抗菌效果同0.5 g.L^-1洗必泰无明显差异。提示五倍子是一种具有应用前景的防龋中药。

Abstract:Objective: To compare the antibacterial effect of Galla Chinensis with that of chlorhexidine by means of chemostat and provide experimental foundation for caries prevention with Chinese medicine instead of chemicals in future.Method: A multispecies consortium biofilm(BF) model was construted in the chemostat.The growth-inhibition on bacterial biofilm coated on HA was investigated by means of the colony-forming units(CFU).Acid-inhibition was inspected by continual pH-recording in the flow cells.And the profile on the biofilm treated by both experimental medicines was observed with the scanning electromicroscopy(SEM).Result: Both of the experimental medicines could inhibit the growth of the BF bacteria.And Galla Chinensis had no obvious influence on the ecological composing of the BF bacteria.Both of the experimental medicines could inhibit the acid producion of experimental bacteria.But the final pH of Galla Chinensis was lower than that of chlorhexidine.A typical biofilm formed on HA was observed by SEM.Both of Galla chinensis and chlorhexidine could inhibit the production of extracellular matrix and make the BF profile on the surface of the HA clearer and simpler.Conclusion: Galla Chinensis had definite antibacterial effect.No obvious difference is found between Galla Chinensis(4 g·L^-1) and chlorhexidine(0.5 g·L^-1).Therefore,it is possible for Chinese medicine to gradually replace the chemical medicine in clinic and it will give a new choice for caries prevention.

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