Abstract:Objective: To confirm the medicinal plants of Gramineae and their distribution in Guiyang city.Method: Through field investigations and comparing the collected speciments and literatures,the geographical distribution and functions of the medicinal species were investigated.Result: 24 new medicinal species of Gramineae were found in Guizhou,while 5 species were absent in Guizhou.And 1 was first recorded for medicinal uses,3 species newly added,and the latin name of 1 species was corrected.Conclusion: There are 72 medicinal species and 4 varieties in Guiyang.