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期 刊 ：中国中药杂志 2005年 30卷 04期 页码：257.

关键词：豨莶草；奇壬醇；TLC；HPLC；

Keywords:Siegesbeckiae, kirenol, TLC, HPLC,

摘 要 ：目的:探讨莶草药材的定性、定量检测方法。方法:采用TLC检定莶草中奇壬醇的含量。结果:以氯仿 甲醇 甲酸(25∶5∶1)为薄层展开系统,可以清晰地检测奇壬醇;采用Polaris C₁₈色谱柱,以乙腈 水(25∶75)为流动相,可使奇壬醇达到基线分离,莶草中奇壬醇平均含量为 0.14%,奇壬醇在0.186~14.88μg呈良好线性关系,平均回收率100 0%,RSD 2.4%。结论:本法可作为莶草药材的定性、定量检测方法。

Abstract:Objective: To establish the qualitative and quantitative methods of Herba Siegesbeckiae. Method: A TLC method was used for qualitative identification and a HPLC analysis was applied for quantitative determination of Herba Siegesbeckiae with kirenol as the reference substances. Result: Chloroform-methanol-formic acid (25∶5∶1) as a mobile phase of TLC, the spot of kirenol can be easily detected; Methanol extracts of Herba Siegebeckiae were separated on a Polaris C₁₈ column with acetonitrile-water(25∶75) as mobile phase and kirenol was separated well. The average content of kirenol in Herba Siegebeckiae was 0.14%. A good linear relationship between the peak areas and injected amounts of kirenol in the range of 0.19-14.9 μg and the arerage recovery was 100.0% (RSD=2.4%). Conclusion: The method can be used for qualitative identification and quantitation determination of Herba Siegesbeckiae.

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