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期 刊 ：中国中药杂志 2005年 30卷 14期 页码：1092.

关键词：骨碎补总黄酮；骨质疏松症；cDNA arry技术；基因；

Keywords:flavonoids of Rhizoma Drynariae, osteoporosis, cDNA array, gene,

摘 要 ：目的:应用cDNA array技术分析骨碎补总黄酮对去卵巢大鼠基因水平的影响。方法:制作去卵巢大鼠模型,24周后,活杀动物,腹主动脉取血6 mL,并取出脊髓4锄,抽提totalRNA,分离mRNA,进行检测。结果:空白对照组血样与模型组的差异为70个基因,空白对照组与骨碎补总黄酮组的差异为9个基因。骨碎补总黄酮灌服后大鼠模型基因过度表达基本恢复正常。该实验cDNA array分析中脊髓基因基本无差异。结论:骨碎补总黄酮灌服6 个月后大鼠模型基因过度表达能基本恢复正常。表明骨碎补总黄酮对去卵巢大鼠基因表达水平有一定的影响。

Abstract:Objective: To study the effects of flavonoids of Rhizoma Diynariae on gene level of rats without ovaries(OP) by technology of cDNA array. Method: The models of rats without ovaries were made After. 24 weeks of treatment, put them to death and sampled 6 ml blood from the abdominal aorta and 4cm spinal cord. Take out the total RNA, then separate the mRNA to check. Result: There is 70 genes difference between the blank control group and model group, 9 genes difference between the blank control group and flavonoids of Rhizoma Drynariae group. The genes over expression of OP rats models regain normal after the models were fed on flavonoids of Rhizoma Drynariae. There are no differences in the spinal cords genes in the cDNA array analysis of this trail. Conclusion: The genes over expression of OP rats models regain normal after the models were fed on flavonoids of Rhizoma Drynariae for 6 months. The result shows that the flavonoids of Rhizoma Drynariae does have certain effects on the gene expression of rats without ovaries

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