Abstract:Objective: To study the chemical constituents in seeds of Helicia nilagirica.Method: The ethanol extract was seperated by petroleum ether, dichloromethane, n-butanol in sequence, then isolated by silica gel column chromatography. The structures were identified and elucidated by physicochemical properties and spectral analysis. Result: Five compounds were isolated from the dichloromethane and n-bu-tanol extracts, identified as p-hydroxybenzaldehyde (1) , p-hydroxybenzoic acid (2) , gallic acid (3) , helicide (4) , 4-formylpyrnyl-O-β-D-glucopyranoside (5) . Conclusion: All the compounds except Ⅳ were isolated from the plant for the first time. The compounds Ⅰ,Ⅱ and Ⅲ were isolated from the genus Helicia for the first time.