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期 刊 ：中国中药杂志 2003年 28卷 02期 页码：133.

关键词：HPLC；养肝口服液；4-四羟基二苯乙烯-2-O-β-D-葡萄糖苷；

Keywords:HPLC, 2, 3, 5, 4‘-tetrahydroxystilben-2-O-β-D-glucoside, Yangan Oral Liquid,

摘 要 ：目的:建立养肝口服液的含量测定方法。方法:样品用乙醇稀释,在C₁₈柱上以乙腈-水(14:86)为流动相,在波长320nm处检测。结果2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷在0.063 6-0.3180μg线性关系良好,r=0.999 8,平均回收率为99.0％,RSD为1.3％。结论:本法灵敏、准确,简便易行,重现性好。

Abstract:Objective: To establish the determination method of 2,3,5,4‘-tetrahydroxystilbene-2-O-β-D-glucoside in Yangan Oral Liquid. Method: 2,3,5,4‘-tetrahydroxystilbene-2-O-β-D-glucoside was determined by HPLC on C₁₈ column,in acetonitrile-water (14:86)solution as a mobile phase,detection wavelength as 320 nm. Result: This method showed a good linear relationship. The average recovery was 99.05% and RSD was 1.31%. Conclusion: The method was simple with styong specificity and good repriducibility and can be used for quality control for Yangan Oral Liquid.

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