Abstract:In this paper, the method for determining genkwanin in Flos Genkwa was established by HPLC. De-tected at 332nm on a Lichrosorb 5 RP-18 column with a mobile phase of methanol-water-acetic acid (65: 35: 5),thecontent of genkwanin in Flos Genkwa was determined to be 0.16%. The recovery rate was 95.46% and RSD1. 15%.