---

期 刊 ：中国中药杂志 1998年 23卷 12期 页码：707.

关键词：杜仲；25SrDNA；序列分析；

Keywords:Eucommia ulmoides, 25S rDNA, sequence analysis,

摘 要 ：目的:为中药杜仲真伪品的鉴别提供新的分子水平的资料。方法:采用末端终止法对中药杜仲大分子rRNA基因(25SrDNA)5’端进行了序列测定。结果:根据序列中核苷酸的变化可有效地鉴别杜仲与其它相关类群。结论:为进一步开展药材杜仲专一性核酸分子探针的研制奠定了基础。

Abstract:Objective:To provide new molecular data for the identification of the Chinese medicinal plant Duzhong(Eucommia ulmoides).Method:Sequencing and analyzing the 25S rDNA gene(rDNA)5terminal region.Result:More than 300 nucleotides of 25S rDNA 5 terminal region were determined,and the specific nucleotides can be used for the identification of this medicinal plant.Conclusion:This sequence analysis may serve as a basis for preparing the specific rDNA probe.

---