Abstract:A method for the determination of plasma concontration of liensinine with HPLC and pharmacokinetic study of liensinine in rabbits were reported. Assay linearity was shown over the range of 0.125～2.5 μg/ml serum with regression coefficient of 0.9-997. The mean recoveries of liensinine in plasma (within-day and between-days) were all more than 99% of the dose and the coefficients of variation were less than 5%. After a single intravenous injection of liensinine 6mg/kg to rabait. The pharmacokinetic characteristics were found to fit a twocompartment open model. Important parameters were: t1/2α=8.303min tl/2β=129.960minAUC=132.67μg. min/ml CL=0.045 L/min Vc=2.768 L/kg.