Abstract:In this paper four compounds isolated from Radix Ophiopogonis are described. Their structures have been identified as β-sitosterol (Ⅰ). 6-aldehydo-7-methoxy-isoophiopogonanone B (Ⅱ), α-sitosterol-β-D-glucopyranoside (Ⅲ) and 1-borneol-β-D-apisyl-β-D-glucopyranoside (Ⅳ) on the basis of spectral data and hydrolytic reaction.