Abstract:Five constituents were isolated from the root of Euphorbia fischeriana.Three of them were-identified as jolkinolide B(D),jolkinolide A(E)and 17-hydroxyjolkinolide B(H),by spectro-scopic and chemical evidence.Structure determination of compounds F and G is still under way.D,E and H were first reported present in the root of Euphorbia fischeriana.