---

作 者 ：韩利文,侯晋军,赵亮,梁泰刚,李青山

期 刊 ：中草药 2008年

关键词：罗布麻叶；HPLC；指纹图谱；鉴别；

Keywords:Folium Apocyni Veneti, HPLC, fingerprint, identification,

摘 要 ：目的建立罗布麻叶的HPLC指纹图谱。用以控制罗布麻叶的内在质量,并对罗布麻叶药材与其混淆品进行鉴别。方法采用HPLC梯度洗脱的方法建立指纹图谱,"计算机辅助相似度评价系统"软件进行数据处理.据此对不同种属的药材进行比较。结果建立的罗布麻HPLC指纹图谱方法的精密度、稳定性和重现性较好。罗布麻叶(红麻叶) HPLC指纹图谱共标定12个共有指纹峰,与白麻属样品图谱之间呈显著差异。可以依此鉴别。结论HPLC指纹图谱可以用作罗布麻叶药材的质量控制。

Abstract:Objective To establish chromatographic fingerprint of Folium Apocyni Veneti by HPLC and identify Folium Apocyni Veneti from its adulterants.MethodsMethods HPLC Gradient elution was applied to establish the chromatographic fingerprint and"Computer-Aided Similarity Evaluation System"was used in data analyses.Results This chromatographic fingerprint method has good precision,stability,and re- peatability.Twelve common peaks were marked.There are notable differences in chromatographic finger- prints between Folium Apocyni Veneti and its adulterants.Conclusion This chromatographic fingerprint method can be used to evaluate the quality of Folium Apocyni Veneti.

---