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作 者 ：林义章,张淑媛,朱海生.

期 刊 ：中国生态农业学报 2008年 16卷 4期 页码：948-951

关键词：小白菜；铜胁迫；叶肉细胞；超微结构；

Keywords:Chinese cabbage, Copper stress, Mesophyll cells, Ultra-structure,

摘 要 ：利用透射电镜技术对铜胁迫下2个小白菜品种（“五月慢”和“上海青”）叶肉细胞超微结构的变化进行了观察。结果表明：未胁迫下，小白菜叶肉细胞中各细胞器结构完整。铜胁迫下，两个小白菜品种单位面积叶绿体数目随铜处理浓度的增大而减少。“五月慢”叶肉细胞超微结构在10 mg·L^{-1、20 mg·L-1铜处理下叶绿体受损较轻，在高铜胁迫（40 mg·L-1、50 mg·L-1）下，叶绿体被膜破损，线粒体嵴减少，出现晶体结构。“上海青”叶肉细胞超微结构随铜浓度的升高损伤不断加重，在高浓度铜胁迫下出现质壁分离，叶绿体内、外膜解体消失，线粒体嵴混乱，有泡状小球出现。以上结果还表明，“五月慢”较“上海青”耐铜。}

Abstract:Ultra-structural changes in mesophll cells of two breeds of Chinese cabbage (“Wuyueman” and “Shanghaiqing”) under copper stress were investigated with transmission electron microscopy. When Chinese cabbage grows in the normal copper condition, various organelles of the crop morphologically integrate. But when it grows under copper stress, the number of chloroplasts reduces with increasing external copper concentration in both breeds. The chloroplast structure in leaf cells of “Wuyueman” is slightly injured when the concentration of Cu²⁺ reaches 10～20 mg·L^{-1. However, chloroplast membrane disrupts, some mitochondria cristae decreases, and certain crystal structure in some leaf mitochondria cells of “Wuyueman” are observed under high concentration of Cu2+(40～50 mg·L-1). In the leaf cells of “Shanghaiqing”, it is observed that ultra structural damage gradually strengthens with increased Cu2+ concentration. The cell wall and cytoplast are separated, the chloroplast membrane is almost disorganized, some mitochondria cristae float in chaos and the vesicular sphere is visible under such concentration. The results also indicate that “Shanghaiqing” is more sensitive to copper stress than “Wuyueman”.}

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