全 文 ：Mycosystema
菌 物 学 报 15 January 2009, 28(1): 056-059

jwxt@im.ac.cn
ISSN1672-6472 CN11-5180Q
©2009 Institute of Microbiology, CAS, all rights reserved.






Supported by Natural Science Research Fund of Guizhou Provincial Education Bureau (No. 2007086), Key Natural Science Research Fund of Kaili
University (No. Z0707), and Key International Science and Technology Cooperation Project of China (No. 2001CB711103) from Ministry of Science
and Technology.
*Corresponding author. E-mail: leiqiyi@126.com
Received: 06-11-2008, accepted: 23-12-2008

Notes on three bolete species from China
LEI Qi-Yi1 ZHOU Jiang-Ju1 WANG Qing-Bin1, 2*
1Department of Biotechnology, Kaili University, Kaili 556000, China
2Key Laboratory of Systematic Mycology and Lichenology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China


Abstract: Three bolete species are discussed. Boletus sinensis is proposed as a new combination of Xerocomus sinensis, B.
borneensis is excluded from bolete flora of China, and B. brevitubus is synonymous to Phlebopus marginatus. Redescriptions are
given for each species.
Key words: Boletales, Boletus, new combination

对三种中国产牛肝菌的研究
雷启义 1 周江菊 1 王庆彬 1,2*
1凯里学院生物科学技术系 凯里 556000
2中国科学院微生物研究所真菌地衣系统学重点实验室 北京 100101
摘 要：对三种中国产牛肝菌进行了重新研究。将中华绒盖牛肝菌 Xerecomus sinensis 移至 Boletus 中，组合为 B. sinensis；
将南亚牛肝菌 B. borneensis 从我国牛肝菌区系中排除；确认短管牛肝菌 B. brevitubus 为 Phlebopus marginatus 的同物异名。
关键词：牛肝菌目，牛肝菌属，新组合

The numbers of boletes in China have increased
significantly since Chiu (1948) reported fifty-seven
species from Yunnan. Many new species, new records,
and even a new genus, Sinoboletus M. Zang (1992), have
been reported (Zang 1980, 1983, 1991; Zang & Petersen
2004; Bi et al. 1982, 1984; Wang et al. 2003). More than
400 taxa of both tubulate and lamellate boletes have been
reported so far (Li & Song 2000). A Chinese checklist of
Boletus Fr. and Xerocomus Quél. has been recently
compiled with annotation on their current status and
known distribution by Wang (2004), and a total of 337
names of Boletus were included according to an
extensive literature search. Of these names, 108 are
recognizable (including two varieties and two
subspecies). However, among the 108 taxa, about 20 can
not be confirmed through specimen examination, and 14
lack the support of specimens. Many taxa should be
re-studied, although Zang (2006) reviewed the progress
of studies in Boletaceae from China and published the
regional monographical works. This paper reports
preliminary progress of revisionary studies on boletes
from China.
DOI:10.13346/j.mycosystema.2009.01.027
Vol.28 No.1 57
http://journals.im.ac.cn/jwxtcn
Boletus sinensis (T.H. Li & M. Zang) Q.B. Wang & T.H.
Li, comb. nov.
Boletus luridus f. sinensis T.H. Li in Bi et al.,
Preliminary Agaric Flora of Hainan Province: 294 (1997),
nom nud.
Xerocomus sinensis T.H. Li & M. Zang, Mycotaxon 80:
486 (2001).
Pileus 4.0−13.0cm in diam., hemisphere, dry when
wet, gray-yellow-brown with olive tint or reddish tint
sometimes, tomentose, margin incurved. Context
0.8−1.5cm thick at the stipe, pale yellow to yellow,
changing blue when bruised, odour and taste mild. Tube
2.5−5.0mm long, yellow, changing blue when bruised,
adnate; pores 1−3/mm, subangular, red to red-brown,
changing blue when bruised. Stipe central, 4.0−12.0cm
long, 1.0−2.5cm thick, enlarging download, red to
purple-red at upper part, and concolorful with pileus
downward, covered with purplish-red reticulation.
Basidiospores 8.0−10.5 × 3.5−5.0µm, fusoid to elliptical,
smooth, pale yellow. Basidia 27−32 × 7.0−9.5µm,
clavate, 4-spored. Pleurocystida 30−55 × 7.0−9.0µm,
fusoid, pale yellow. Tube trama Boletus-type, divergent
from central strand. Pileipellis trichodermium, hyphae
yellow to yellow-brown. Clamp connections absent.
Specimen examined: Hainan Province: Ledong
County, Jianfeng Ling, HMIGD 13070 (Holotype,
deposited in Herbarium of Microbiology Institute of
Guangdong).
Habit and Habitat: Scattered to gregarious on
ground in mixed woods.
Distribution: Hainan Province, China.
Boletus sinensis is obviously close to B. luridus
Schaeff., which has yellow to olive-yellow pileus, red to
orange-red hymenophore and red reticulation on the stipe
(Bessette et al. 2000). The spore size of B. luridus varies
in related literatures, e.g. 11−15 × 4.5−6.5µm (Watling
1970), 12−17 × 5.5−7µm (Smith & Thiers 1971), 11−15
× 5−6.5µm (Grund & Harrison 1976), 11−16 × 5−7µm
(Bessette et al. 2000). For Chinese specimens, the spores
of this species were measured as 10−13 × 3.5−4.5µm by
Teng (1963), 8−12 × 4−6µm by Bi et al. (1990, 1994),
11−14 × 5−6µm by Mao et al. (1993) and Mao (1998,
2000), and 12−17 × 5.5−7µm by Ying & Zang (1994)
and Yuan & Sun (1995). All of these measurements are
larger than spore measurements of B. sinensis. Therefore,
the spore sizes can distinguish B. sinensis from B. luridus
definitely.
Zang et al. (2001) elevated B. luridus f. sinensis to
species level and transferred it to Xerocomus, but the
species has a red to red-brown hymenophore, a typical
character of Boletus sect. Luridi (Singer 1986), thereby,
the new combination is made herein.
Boletus borneensis auct. non Corner: Zang et al., Acta
Mycologica Sinica 12: 279, 1993.
Specimen examined: Guangdong Province: Suixi
County, 17 June 1991, M.Q. Gong No. 9101, HKAS
23433.
Habit and Habitat: Solitary to scattered on the
ground, under Eucalytus sp. tree.
B. borneensis is firstly reported in China without
description from Guangdong Province by Zang et al.
(1993), and recorded again by Zang (2006).
Re-examination on the specimen (HKAS 23433) cited by
Zang et al. (1993) and Zang (2006) shows that the pileus
is umber to ochraceous, smooth and the fungus is similar
to B. borneensis described by Corner (1972). However,
the stipe is enlarged downward, reticulate at least on the
top, smooth or slightly striate, and obviously different
from that described by Corner (1972). Corner (1972)
described the stipe as wholly coarsely scurfy-squamulose.
The difference in stipe character can discriminate them
unambiguously. On the other hand, the spores of the
Chinese specimen are 11.0–14.5 × 3.5–4.5µm in size,
elliptical, olive-brown, and obviously smaller than those
examined by Corner (1972), which are 14–16 (–18) ×
4–5 (–5.5)µm and pale vinaceous-chocolate. It is obvious
that the fungus reported from China lacks typical
morphological characters of B. borneensis. Therefore,
the distribution of B. borneensis in China can not be
confirmed according to present data. The fungus may be
close to B. pulverulentus Opat.
Phlebopus marginatus (Berk.) Watling & N.M. Greg. in
Proc. R. Soc. QD 99: 67, 1988.
Phaeogyroporus portentosus (Berk. & Broome) McNabb
in N.Z. Jl. Bot. 6: 142, 1968.
Phlebopus portentosus (Berk. & Broome) Boedijn in
Sydowia 5: 218, 1951.
Boletus portentosus Berk. & Broome in J. Linn. Soc.,
Bot. 14: 46, 1873.
Boletus brevitubus M. Zang in Acta Mycol. Sin. 10: 117,
58 Mycosystema

1991.
Pileus 7.0−22cm in diam., hemisphere to subconvex,
depressed at centre, deep brown or black-brown to
almost black, glabrous, slightly viscid when wet. Context
2.0−3.0cm thick, dull yellow when young, becoming dull
whitish when old, unchanging when bruised, odour mild,
taste slightly acid. Tube 5.0−7.0mm long, dull
greenish-yellow, unchanging when bruised, adnate
around the stipe; pores 2−3/mm, minute, round,
black-brown when young, becoming deep brown
overnight, unchanging or just intensifying. Stipe
9.0−10.0cm long, 4.0−4.5cm thick, robust, enlarging
downward, dull brown at the apex, dark to black-brown
downward, tomentose or pruinose, mycelium pale yellow
at the base, context concolorous with that of pileus,
unchanging when bruised. Basidiospores 7.0−11.0 × (5.5)
6.0−7.0 (−8.0)µm, broadly elliptical to almost round,
smooth, thin-walled, brown in KOH. Basidia 19.0−37 ×
8.0−10.0µm, short clavate. Tube trama typical
Boletus-type, divergent from center grand, hyphae
hyaline with clamp connections.
Specimens examined: Yunnan Province: Jinghong
County, 3 Aug. 2003, Q.B. Wang No.132, HMAS 76825;
Menglun, Tropic Forest Garden of Xishuangbanna,
Chinese Academy of Sciences, under Quercus sp. tree, 5
Aug. 2003, Q.B. Wang and M. Li No.163, HMAS 79592,
ibid, 8 Aug. 2003, Q.B. Wang No.178, HMAS 76824;
ibid, 30 Oct. 1989, ZhuL. Yang No. 877, HKAS 22180;
Mengla market, 7 Aug. 2003, Q.B Wang & T.Z. Wei No
173, HMAS 76823; Menglun, Yitan Garden, under the
mixed woods, 7 Aug. 2003, Q.B. Wang No.174, HMAS
79620; Yuanyang County, Nanshahe, 11 Sep. 1973, Q.T
Zhang & H. Li, HKAS 516 (Holotype).
Habit and Habitat: Solitary to scattered on the
ground, under Quercus sp. tree.
Distribution: Yunnan Province, China.
Phlebopus marginatus is a tropical species. Zang
(1986) suspected that this species (under the name B.
portentosus) might be distributed in south of Yunnan
Province. Zang et al. (1993) reported this species as B.
portentosus and listed P. portentosus as a synonym, and
then Zang (1999, 2006) reported successively it again.
Re-examination on HKAS 23441 cited by Zang et al.
(1993) shows that the pileus is slightly viscid,
cinnamon-brown to deeply red-brown; pores are minute
and adnate, yellow-brown; stipe is equal, solid
chestnut-brown; the spores are 9.0−10.0 × 3.0−4.0µm,
olive-brown, elliptical to fusoid; pleurocystidia are
28−36 × 8.0-10.0µm, inflated at center, tube trama is of
Boletus-type, divergent from the center strand and clamp
connections are absent. These macro- and micro-
characters imply that it does not fit the concept of P.
marginatus well, especially the spore size and the
absence of clamp connections. The so-called ‘B.
portentosus’ reported from China possesses characters
different from the typical morphology of P. marginatus
and the fungus possibly resembles Suillus pinetorum (W.
F. Chiu) H. Engel & Kloface. The present collections of
P. marginatus cited above are therefore the first evidence
of the occurrence of the species from China.
Boletus brevitubus M. Zang (1991), a species
reported from south Yunnan, is tropical, associated with
Cephalocitrus grandis (Obs.) Tseng and Delonix regia
(Bojer) Rafin. It is mainly characterized by brown,
black-brown to black pileus, minute pores and short
tubes, and ovoid to broadly elliptical spores. All these
characters correspond to those of P. marginatus well.
Re-examinations on the specimens HKAS 516 (holotype)
and HKAS 22180 cited by Zang (1991) shows that the
spores of the former are 8.0−10.0 × 5.5 −6.5 (−7.0)µm,
and those of the latter are 7.0−11.0 × (5.5) 6.0 −7.0
(−8.0)µm, both are almost the same as those of P.
marginatus. Clamp connections are observed in both of
the specimens. Notes on the label of collection HKAS
22180 made by Zhu L. Yang (the collector) are: ‘the
pileus 6.0−8.0cm in diam., dark brown, tomentose,
hymenophore yellow at first then dull brown,
unchanging when bruised, stipe robust, enlarged
downward, 2.0−4.0cm thick, 5.0−7.0cm long, tomentose,
dull brown to nearly dark brown’(Notes on the label of
collection HKAS 22180 made by Zhu L. Yang). These
characters are identical to those of our specimens. HKAS
22180 and our specimens are all collected at Tropic
Forest Garden of Xishuangbanna, Chinese Academy of
Sciences. We think there are no significant differences
between B. brevitubus and P. marginatus and they are
conspecific.
Phlebopus, a small genus with 12 species (Kirk et al.
2001), is mainly distributed in pantropical and south
temperate region (Watling & Gregory 1988). In China, P.
Vol.28 No.1 59
http://journals.im.ac.cn/jwxtcn
marginatus is known only in Xishuangbanna, Yunnan
Province at present.

Acknowledgements: The authors are thankful to Prof. T.H. Li,
Guangdong Institute of Microbiology, for amending the
manuscript of this paper, and to Prof. P.G. Liu and Dr. Zhu L.
Yang, Kumming Institute of Botany, Chinese Academy of
Sciences, for their kind help in field works. We are grateful to
Mr. L.S. Wang, curator of HKAS, for the loan of specimens.


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