全 文 ：resistance was controlled by a single dominant gene.Characteri-
zation of these w heat lines was car ried out by GISH and RFLP
analysis.The results o f GISH show ed that the lines , Yw 642 ,
Yw443 and Yw243 etc., were homozygous wheat- Th .inter-
medium translocation lines containing 20 pairs of w heat chromo-
somes and 1 pair of w heat-Th.intermedium translo cation chro-
mosomes , in w hich the chromosome segments of Th intermedi-
um were transferred to the distal end o f a pair of w heat chromo-
somes.RFLP analysis indicated that the translocation chromo-
some of the w heat lines was T7DS·7DL-7XL transloca tion.The
breakpoint of translocation is lo cated on the distal end of 7DL ,
between Xpsr965 and Xpsr680 , about 90-99 cM from the cen-
tromere.The BYDV gene is loca ted on the distal end of 7XL
around Xpsr680 , Xpsr687 and Xwg380.The RFLP markers of
psr680 , psr687 and w g380 co-seg regated with the BYDV resis-
tance and could be used for marker-assisted selection(MAS)in
wheat breeding progr am for BYDV resistance.
Key words:Thinopyrum intermedium , BYDV , disease resis-
tance , translocation , GISH , RFLP , homoeolog ous g roup 7
多枝赖草 DNA导入小麦引起重要农艺性状变化及相应的分子证据
李维琪 ,等
(中国科学院新疆化学研究所 ,乌鲁木齐 830000)
关键词:小麦;多枝赖草 DNA;农艺性状
中图分类号:Q　　　文献标识码:A　　　文章编号:0253-9772(2001)-01-0052-02
Important Agronomic trait Changes in Wheat Caused
by Introduction of Leymus racemoses DNA and Some Molecular Proofs
LI Wei-qi ,ZHAO M in-an , LIU Yun-ying ,M IAO Jun , LIU Min
(Xinj iang Inst i tute of Chemistry , The Chinese Academy of S ciences , U rumqi , 830011)
QI Jia-hua ,ZHANG M ao-y in ,WANG Zi-xia ,Haizigoli Yang Ke-rui
(Insti tute of Nuclear and Biotechnology , X inj iang Academy of Agricul tural S ciences , Urumqi , 830000)
　　In 1989 , Leymus racemoses DNA was introduced
into a spring w heat cult ivar “ 761” through pollen
tube Pathw ay .In the resulted F3 progenies , appar-
ent t rait changes w ere observed.In the F4 progenies ,
many lines , possessing longer ears , more g rains per
ear , higher 1000 grain weight and higher protein
content , were obtained.We reasoned that some ge-
netic components of Leymus racemoses might have
been transferred and integ rated into the w heat
genome.In order to get molecular proof for above
reasoning , RFLP , RAPD and storage pro tein analy sis
w ere carried out.The following results w ere ob-
tained.1.RFLP analysis.Four repetitive DNA se-
quence (pHv7161 , pHv7179 , pHv7191 and
pHv7293)clones from barley (Hordeum vulgare)
genome w ere used for molecular hybridization using
the genome DNA of Leymus racemoses(donor),
spring wheat 761 (receptor)and the putative trans-
formed wheat lines.Our results revealed some bands ,
common to Leymus racemoses and the putative trans-
formed wheats , were absent in spring wheat 761.2.
RAPD analysis.160 operon primers w ere used to am-
plify polymorphic bands using genomic DNA of Ley-
mus racemoses , spring w heat 761 and the putative
t ransformed wheats.20% of the primers w as poly-
morphic.The calculated genetic relationship based on
the RAPD analysis w ere:35%betw een Leymus race-
moses and spring w heat 761 , 90%between the puta-
tive t ransformed wheats and spring w heat 761.3.
Storage protein analysis.After comparison of the pat-
52 　　　　　　　　　　　　　　　　　遗　传 HEREDITAS(Beijing)　2001　　　　　　　　　　　　　　　23卷
DOI :10.16288/j.yczz.2001.01.028
terns of gliadin and glutenin by SDS-PAGE among
Leymus racemoses , spring w heat 761 and the putative
transformed wheats , we found that , in gliadin analy-
sis , a new band appeared in the putative t ransformed
wheats with an electropho retic mobility similar to
that of a gliadin polypeptide of Leymus racemoses.In
glutenin analy sis , a few high molecular weight
glutenin subunits appeared in the putative trans-
formed wheats , which might be derived f rom Ley-
mus racemoses.These subunits may have direct im-
pact on f lour processing properties , and are worthy of
further investigations.
利用异源双代换系杂交产生染色体易位系
贾　旭
(中国科学院遗传研究所植物细胞与染色体工程国家重点实验室 ,北京 100101)
关键词:双代换系;染色体;易位系
中图分类号:Q343　　　文献标识码:A　　　文章编号:0253-9772(2001)-01-0053-01
Production of Chromosome Translocation via Crossing
Two Different Alien Substitution Lines in Wheat
JIA Xu
(The S tate Key Laboratory of Plant Cel l and Chromosome Eng ineering ,
Insti tute of Genetics , The Ch inese Academy of Sciences , Beij ing 100101 , Ch ina)
　　T ranslocation lines with useful genes derived from wheat
related species are valuable in w heat v ariety improvement.But it
is no t easy to produce them.The frequency of translocation line
production is usually low in w heat.So far , most of translocation
lines have been obtained by spontaneous translocation method.
It was once suggested that univalent chromosomes would mis-di-
vide, reunion , and form chromosome translocation at meiosis.
This theory has been supported by some experiments.We de-
signed a series of experiments to increase univalent number at
meiosis to test if univ alent chromosome number w as related to
translo cation frequency.Our results show ed that crossing two
different w heat-alien substitution lines could indeed increase the
frequency of chromosome translocations.
过去 50年中中国小麦品种在 Glu-A1 , Glu-B1
和 Glu-D1 位点上等位基因的变化
张学勇 ,董玉琛
(中国农业科学院品种资源研究所 ,北京 100081 , China)
关键词:小麦;基因
中图分类号:Q943　　　文献标识码:A　　　文章编号:0253-9772(2001)-01-0053-02
531 期　　　　　　　　　　　　　李振声等:小麦分子与细胞遗传学研讨会论文摘要