全 文 ：农业生物技术学报
Journal of Agricultural Biotechnology
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拟南芥细胞核内 Phytochrome Interacting Factor 蛋白可通过 COP1 E3 连接酶
促进光敏色素 B的聚泛素化
Arabidopsis Phytochrome Interacting Factor Proteins Promote Phytochrome B
Polyubiquitination by COP1 E3 Ligase in the Nucleus
植物体的许多光响应如：萌发、避荫反应、开花等均由光敏色素 B（phyB）介导。黑暗条件下，phyB以非生理活性的 Pr形式
存在于细胞质中，经红光照射后，phyB转变为具有生理活性的 Pfr形式，由细胞质转运到细胞核，引发下游光信号反应，phyB的
降解可能对该信号反应的终止起到调控作用，但其生理机制尚不明确。Nam-Hai Chua等通过研究表明，COP1是 phyB和 phyD
等类型Ⅱ光敏色素的 E3连接酶，揭示了 COP1作为 E3连接酶对光信号途径的调控机制，并发现了 Phytochrome Interacting
Factor（PIF）蛋白作为该途径负调控因子的作用机制。这一研究结果发表在 2010年 6月出版的《Plant Cell》杂志上。
研究人员以拟南芥（Arabidopsis thaliana）为材料，通过 Co-IP和体外泛素化分析等实验方法研究发现，在细胞核中 COP1作
为 E3连接酶，可与 phyB的 N端直接作用，使其发生聚泛素化，然后由 26s蛋白酶体降解，从而调控植物体 phyB等类型Ⅱ光敏
色素的水平，进而调控下游光信号反应。在这个信号途径中，PIFs可以增强 phyB/COP1二者之间的相互作用，促进 phyB聚泛
素化和降解，通过这一机制对 phyB光信号途径的关键步骤起到调控作用。
编者：金京波（中国科学院植物研究所），本刊通讯员
本文引用格式：金京波, 2010,拟南芥细胞核内 Phytochrome Interacting Factor蛋白可通过 COP1 E3连接酶促进光敏色素 B的
聚泛素化,农业生物技术学报, 18(6): 1122
信息来源：http://www.plantcell.org/cgi/content/full/22/7/2370
1122