全 文 ：磷肥和石灰对红壤白三叶草、
大翼豆和圆叶决明生长的影响
董春华 1，2，3，刘 强 1，文石林 2，3*，曾希柏 4，徐
震 1，高菊生 2，3 （1.湖南农业大学资源环境学
院，湖南长沙 410128；2.中国农业科学院农业
资源与农业区划研究所，祁阳农田生态系统国
家野外试验站，湖南祁阳 426182；3.中国农业
科学院农业资源与农业区划研究所，农业部植
物营养与养分循环重点开放实验室，北京
100081；4.中国农业科学院农业环境与可持续
发展研究所/农业部农业环境与气候变化重点
开放实验室，北京 100081）
摘 要 [目的]研究磷肥和石灰对红壤白三
叶草、大翼豆和圆叶决明生长的影响，为推广
白三叶草、圆叶决明和大翼豆的栽培提供理论
依据。[方法]以白三叶草、大翼豆和圆叶决明
为材料，于 2010年采用土培盆栽试验，研究南
方丘陵区酸性红壤上石灰和磷肥配合施用对
其生长发育的影响。[结果]同一石灰用量下，
施磷能明显提高供试牧草株高、总分蘖数和植
株干物质量。不施磷处理下，1.4 g/kg土的石
灰用量下 3种牧草植株总生物量最高，对红壤
酸性改良效果最好；施 200 mg/kg 土磷肥处
理下，2.1 g/kg土的石灰用量下白三叶草和大
翼豆植株总生物量最高，1.4 g/kg 土的石灰用
量下圆叶决明植株总生物量最高。[结论]该试
验结果对白三叶草、大翼豆和圆叶决明在南方
丘陵区酸性红壤上的栽培有重要的指导和实
践意义。
关键词 白三叶草；大翼豆；圆叶决明；磷肥；
石灰
基金项目 国家科技支撑计划“中低产田改良
科技工程”项目（2012BAD05B05）；农业部公益
性行业科研专项经费项目（201303139）资助。
作者简介 董春华（1976-），男，湖南祁阳人，
助研，博士，主要从事土壤改良与植物营养研
究，E-mail: dch200707@yahoo.com.cn。* 通讯
作者，E-mail：dch1001@126.com。
收稿日期 2013-02-10
修回日期 2013-03-25
Effects of Phosphate and Lime Application on
Growth of Trifolium repens, Chamaecrista
rotundifolia and Macroptilium atropurpureum in
Red Soils
Chunhua DONG1,2,3, Qiang LIU1, Shilin WEN2,3*, Xibai ZENG4, Zhen XU1, Jusheng GAO2,3
1. College of Resource and Environment, Hunan Agricultural University, Changsha 410128, China;
2. Institute of Agricultural Resources and Regional Planning CAAS, Qiyang Agro-ecosystem of National Field Experimental Sta-
tion, Qiyang 426182, China;
3. Institute of Agricultural Resources and Regional Planning CAAS, Key Laboratory of Plant Nutrition and Nutrient Cycling of
Ministry of Agriculture, Beijing 100081, China;
4. Institute of Agricultural Environment and Sustainable Development CAAS/Key Laboratory of Agro -Environment and Climate
Change, Ministry of Agriculture, Beijing 100081, China
Supported by National Science & Technology Pillar Program (2012BAD05B05); Special
Fund for Agro-scientific Research in the Public Interest (201303139).
*Corresponding author. E-mail: dch1001@126.com
Received: February 10, 2013 Accepted: March 25, 2013A
Agricultural Science & Technology, 2013, 14(4): 640-644
Copyright訫 2013, Information Institute of HAAS. All rights reserved Soil and Fertilizer
Abstract [Objective] The aim was to research effects of P fertilizer and lime on
growth of Trrifolium repens, Chamaecrista rotundifolia and Macroptilium atropur-
pureum, to provide references for cultivation of the three plants. [Method] Pot ex-
periments were conducted with Trrifolium repens, Chamaecrista rotundifolia and
Macroptilium atropurpureum in 2010 in order to research effects of lime and P fer-
tilizer mixture on growth of the plants in southern hilly acidic red soils. [Result] With
lime amount fixed, application of P fertilizer would enhance plant height, total tiller
number and dry matter. When P fertilizer was not applied, however, plant height of
the three plants achieved the peak by lime at 1.4 g/kg which proved best for im-
provement of acidity of red soils. With P fertilizer at 200 mg/kg was applied,
biomass of Trifolium repens and Macroptilium atropurpureum achieved the highest by
lime at 2.1 g/kg, but total biomass of Chamaecrista rotundifolia was the highest by
lime at 1.4 g/kg. [Conclusion] The research provides references for planting and pro-
duction of Trifolium repens, Chamaecrista rotundifolia and Macroptilium atropur-
pureum in southern hilly regions.
Key words Trifolium repens; Macroptilium atropurpureum; Chamaecrista rotundifolia;
P fertilizer; Lime
R ed soils in hilly region in Hu-nan Province are dominatedby fourth quarter red clay
soils, which have good phosphorus
fixation capacity and contain lower
contents of available P and K. In fact, it
is a kind of acidic and infertile soil [1].
Hence, P fertilizer and lime become a
good choice to improve fertility of red
soils[2-4].
Trrifolium repens L., native to Eu-
rope, is widely grown in southern re-
gion in China as a pasture crop[5]. It is
proved by many researches[6-8] that the
plant is sensitive to lime in acidic envi-
ronment. Chamaecrista rotundifolia
(Pers.) Greene is perennial tropical
plant of Fabaceae, which is suitable to
be extensively grown in the south of
China, for some outstanding quality,
such as acidity-resistance, unproduc-
tivity-resistance, and excellent quality
and high yield [9]. The research made
by Zhang et al. [9] suggested that yield
of Chamaecrista rotundifolia was not
enhanced significantly by lime or
ardealite, individually, but doubled by
the mixture of the two. Macroptilium
atropurpureum (DC.) Urban is a climber
in the pea family (Fabaceae), widely
DOI:10.16175/j.cnki.1009-4229.2013.04.019
Agricultural Science & Technology2013
Table 1 Plant height of Trifolium repens, Macroptilium atropurpureum and Chamaecrista
rotundifolia treated by P fertilizer and lime cm
Lime
amount
mg/kg soil
P in Trifolium
repens∥mg/kg soil
P in Macroptilium
atropurpureum∥mg/kg soil
P in Chamaecrista
rotundifolia∥mg/kg soil
0 200 0 200 0 200
0 14.5 c 18.6 b 48.0 b 85.1 a 27.9 bc 39.9 a
0.7 18.8 ab 20.4 ab 66.6 ab 92.9 a 29.4 b 36.8 a
1.4 20.1 a 21.3 a 76.3 a 98.2 a 32.8 a 36.4 a
2.1 18.2 ab 22.3 a 58.2 ab 88.4 a 25.3 c 35.0 a
2.8 15.7 bc 21.4 a 50.9 b 86.6 a 25.0 c 34.2 a
Different lower cases in lines represent differences at 5% level. Similarly hereinafter.
planted in the tropics and subtropics
for grassland improvement and water
maintenance [10 ] . The research con -
ducted by Huang et al.[11] indicated that
the mixture of P and S fertilizers, in-
stead of one of them alone, would im-
prove yield of Macroptilium atropur-
pureum. However, few researches
were made on effects of lime and P
fertilizer on growth of Trifolium repens,
Chamaecrista rotundifolia and
Macroptilium atropurpureum in acidic
soils.
In the research, effects of P fertil-
izer and lime on growth of Trifolium
repens, Chamaecrista rotundifolia and
Macroptilium atropurpureum in red
soils in southern hilly region were ex-
plored in order to seek the optimum of
the two products on the plants to pro-
vide references for planting and ex-
tension of suitable pasture crops in red
soils.
Materials and Methods
Test materials
The test materials included Trifoli-
um repens, Chamaecrista rotundifolia
and Macroptilium atropurpureum, pro-
vided by Red Soil Experimental Sta-
tion in Chinese Academy of Agricul-
tural Sciences. The soils are acidulat-
ed red soils in pinewood. Specifically,
the value of pH is 4.16; content of acti-
vated aluminium is 2 938.2 mg/kg; the
content of total N, total P and total K
are 0.98, 0.29 and 9.6 g/kg; N, P and K
in the test were CO (NH2)2, NaH2PO4
and K2SO4 and lime accordingly.
Test design
The test was conducted in Red
Soil Experimental Station in Chinese
Academy of Agricultural Sciences as
per pot culture. The station is located
in 111° 52′ 32″ E and 26° 45′ 42″ N.
Furthermore, the elevation is in the
range of 150 to 170 m; annual tem-
perature averages 17.8 ℃ ; annual
rainfall is 1 290 mm and annual illumi-
nation is 1 613 h. As per randomized
block design, P concentration included
two levels at 0 and 200 mg/kg; lime
amount was designed at 0, 0.7, 1.4,
2.1 and 2.8 g/kg, respectively. Then, P
would be applied after prepared as so-
lution and lime would be applied di-
rectly. The test, with three repetitions,
involved 10 treatments and every
treatment contained P amount and
lime amount. In addition, three plants
in the test totaled 90 pots.
Plastic buckets at 17 cm ×18 cm
were used in the test and every bucket
contained sieved (5 mm) air-dried soils
at 2 kg. In detail, N at 0.100 g/kg and K
at 0.200 g/kg were applied once for a
time as base fertilizer. On April 24,
2010, 10 seeds of Macroptilium atrop-
urpureum, 20 seeds of Trifolium
repens and 10 seeds of Chamaecrista
rotundifolia were sown. After seedlings
grew, 5 plants of Macroptilium atrop-
urpureum, 10 plants of Trifolium
repens and 5 plants of Chamaecrista
rotundifolia were kept in every pot. On
the 70th d, the plants were harvested
and some items were measured, in-
cluding plant height, number of
branch, dry weight of ground and un-
derground parts of plant.
Measured methods
Total N in soils was measured
with Kjeldahl method; total P was with
sodium hydroxide by Colormetry; total
K was with alkali fusion-flame photom-
etry; activated aluminium was with
volumetric method[12]; plant height was
measured with a meter ruler; tiller nu-
mber was computed; ground and un-
derground parts of plants were wei-
ghed with a scale at 0.01 g.
Statistical work and Analysis
Test data were processed with
Microsoft Excel 2003, analyzed with
SPASS 13.0 and multiply compared
with LSD method.
Results and Analysis
Plant height of Trifolium repens,
Chamaecrista rotundifolia and
Macroptilium atropurpureum treat-
ed by P fertilizer and lime
Without P fertilizer, plants height
of the three plants all increased signifi-
cantly after lime at 0-1.4 g/kg was ap-
plied and dramatically lowered by lime
at 1.4-2.8 g/kg (P<0.05) on the 70th d
of germination (Table 1). These indi-
cated that 1.4 g/kg of lime proves best
in improving the plants in red soils,
without P fertilizer.
With P fertilizer applied, plant
height of Trifolium repens enhanced
dramatically with 0 -2.1 g/kg of lime,
but declined with 2.1-2.8 g/kg of lime;
plant height of Macroptilium atropur-
pureum kept increasing with 0-1.4 g/kg
of lime, but lowered with lime at 1.4-
2.8 g/kg; the plant height of Chamae-
crista rotundifolia kept declining with
lime at 0 -2.8 g/kg, with little differ-
ences. These indicated that when 200
mg/kg of P fertilizer was increased to
be applied in red soils, lime has in-
significant effects on growth of
Macroptilium atropurpureum and
Chamaecrista rotundifolia, and the ef-
fects would be insignificant on Trifoli-
um repens either if lime exceeded 0.7
g/kg.
With the quantity of lime fixed,
plant height was higher in the group
applied with P fertilizer, suggesting
that P fertilizer would promote growth
of the three plants in red soils.
Total tiller number of Trifolium
repens, Macroptilium atropurpur-
eum and Chamaecrista rotundifolia
treated by P fertilizer and lime
Without P fertilizers, tiller number
of Trifolium repens increased dramati-
cally on the 70th d of germination with
lime at 0-2.1 g/kg (Table 2) and low-
ered sharply by lime at 2.1 -2.8 g/kg;
tiller number of Macroptilium atropur-
pureum increased significantly by lime
at 0-0.7 g/kg, but declined remarkably
by lime at 0.7-2.8 g/kg; tiller number of
Chamaecrista rotundifolia increased
little by lime at 0-1.4 g/kg, but declined
remarkably by lime at 1.4-2.8 g/kg.
641
Agricultural Science & Technology 2013
Table 2 Total tiller number of Trifolium repens, Macroptilium atropurpureum and
Chamaecrista rotundifolia treated by P fertilizer and lime
Lime
amount
mg/kg soil
P in Trifolium
repens∥mg/kg soil
P in Macroptilium
atropurpureum∥mg/kg soil
P in Chamaecrista
rotundifolia∥mg/kg soil
0 200 0 200 0 200
0 54.0 b 84.3 b 4.3 b 8.7 a 8.7 ab 13.3 a
0.7 76.0 a 92.0 ab 7.3 a 9.0 a 9.7 ab 14.7 a
1.4 76.7 a 100.7 ab 6.7 a 9.7 a 10.7 a 13.3 a
2.1 78.0 a 106.3 a 5.3 ab 8.7 a 8.7 ab 12.7 a
2.8 51.0 b 90.7 ab 4.0 b 7.3 a 8.3 b 12.3 a
Table 3 Biomass of Trifolium repens, Macroptilium atropurpureum and Chamaecrista
rotundifolia treated by P fertilizer and lime
Lime
amount
mg/kg soil
P in Trifolium repens∥mg/kg soil
Ground part Underground part Total biomass
0 200 0 200 0 200
0 0.35 b 1.25 c 0.04 c 0.12 b 0.39 b 1.37 c
0.7 1.00 a 1.55 bc 0.11 ab 0.14 ab 1.11 a 1.69 bc
1.4 1.10 a 2.02 ab 0.15 a 0.20 a 1.25 a 2.22 ab
2.1 1.05 a 2.11 a 0.12 ab 0.20 a 1.17 a 2.31 a
2.8 0.42 b 1.89 ab 0.06 bc 0.14 ab 0.48 b 2.03 ab
Lime
amount
mg/kg soil
P in Macroptilium atropurpureum∥mg/kg soil
Ground part Underground part Total biomass
0 200 0 200 0 200
0 1.07 b 3.07 a 0.07 b 0.35 a 1.14 b 3.42 a
0.7 1.95 ab 3.70 a 0.14 ab 0.33 a 2.09 ab 4.03 a
1.4 2.58 a 3.86 a 0.18 a 0.46 a 2.76 a 4.32 a
2.1 1.86 ab 4.13 a 0.12 ab 0.35 a 1.98 ab 4.48 a
2.8 1.19 b 4.12 a 0.09 b 0.35 a 1.28 b 4.47 a
Lime
amount
mg/kg soil
P in Chamaecrista rotundifolia um∥mg/kg soil
Ground part Underground part Total biomass
0 200 0 200 0 200
0 1.15 b 2.20 a 0.12 a 0.06 a 1.27 b 2.26 a
0.7 1.27 ab 2.41 a 0.12 a 0.08 a 1.39 ab 2.49 a
1.4 1.42 a 2.42 a 0.14 a 0.08 a 1.56 a 2.5 a
2.1 0.76 ab 2.25 a 0.10 a 0.05 a 0.86 b 2.3 a
2.8 0.75 b 1.95 a 0.09 a 0.06 a 0.84 b 2.01 a
With P fertilizer applied, tiller
number of Trifolium repens grew sig-
nificantly on the 70th d of germination
with lime at 0 -2.1 g/kg, but dropped
sharply with lime at 2.1-2.8 g/kg; tiller
number of Macroptilium atropurpure-
um increased by lime at 0 -1.4 g/kg,
but decreased by lime at 1.4-2.8 g/kg;
tiller number of Chamaecrista rotundi-
folia enhanced remarkably by lime at
0-0.7 g/kg, but decreased by lime at
0.7-2.8 g/kg. These indicated that lime
has insignificant effects on tiller effect
of Macroptilium atropurpureum and
Chamaecrista rotundifolia when P fer-
tilizer increases in red soils.
With lime amount fixed, tiller num-
ber in the group treated with P fertilizer
was significantly higher compared with
group without P fertilizer, illustrating
that P fertilizer would promote tiller ef-
fect of the three plants in red soils.
Biomass of Trifolium repens, Macr-
optilium atropurpureum and Cham-
aecrista rotundifolia treated by P
fertilizer and lime
Without P fertilizer, total dry mat-
ters in underground parts of the plants
changed similarly. Specifically, dry
matter changed from increasing to
decreasing with the peak at 1.4 g/kg.
Yield of Chamaecrista rotundifolia dif-
fered insignificantly. With P fertilizer
applied, the change of dry matter still
kept similar and variations of Macrop-
tilium atropurpureum and Chamae-
crista rotundifolia were insignificant
(Table 3).
Without P fertilizer applied, total
dry matters in underground parts of
the plants also changed similarly.
Specifically, dry matter changed from
increasing to decreasing with the peak
at 1.4 g/kg. With P fertilizer applied,
total amount of dry matter in Trifolium
repens enhanced remarkably by lime
at 0-2.1 g/kg, but began to decline by
adding lime. On the other hand, un-
derground dry matters of Macroptilium
atropurpureum and Chamaecrista ro-
tundifolia were less influenced by lime
with P fertilizer applied.
Regardless of application of P fer-
tilizer, total amount of dry matter in the
three plants changed similarly with that
in underground parts, which coincided
with statistical work. These suggested
that 1.4 g/kg of lime is optimal at grow-
ing the three plants in red soils if P fer-
tilizers are not applied and lime appli-
cation has insignificant effects on dry
matter in Macroptilium atropurpureum
and Chamaecrista rotundifolia if P fer-
tilizers are applied, when 2.1 g/kg of
lime proves optimal to improve Trifoli-
um repens.
Root shoot ratio of Trifolium
repens, Macroptilium atropur-
pureum and Chamaecrista rotundi-
folia treated by P fertilizer and lime
Root shoot ratio is an index to
evaluate growth relation of ground and
underground parts of plants. As shown
in Table 4, when P fertilizer was not
applied, the ratio was higher of Trifoli-
um repens and Chamaecrista rotundi-
folia with lime amount fixed; when P
fertilizer applied, however, the ratio
was increasing in general, indicating
that the nutritional environment has im-
proved to much extent. With lime
amount fixed, the ratio of Chamae-
crista rotundifolia was higher in the
group applied with P fertilizer, com-
pared with that in group without P,
which demonstrated that P fertilizer at
200 mg/kg could not meet demands of
Macroptilium atropurpureum growth
and more nutrients should be supplied
by stronger roots. The change of root
shoot ratio showed that pasture crop
has a good self-adjustment capacity,
which would control more photosyn-
642
Agricultural Science & Technology2013
Table 4 Root shoot ratio of Trifolium repens, Macroptilium atropurpureum and
Chamaecrista rotundifolia treated by P fertilizer and lime
Lime
amount
mg/kg soil
P in Trifolium
repens∥mg/kg soil
P in Macroptilium
atropurpureum∥mg/kg soil
P in Chamaecrista
rotundifolia∥mg/kg soil
0 200 0 200 0 200
0 0.114 0.096 0.065 0.114 0.104 0.027
0.7 0.11 0.09 0.072 0.089 0.094 0.033
1.4 0.136 0.099 0.07 0.119 0.099 0.033
2.1 0.114 0.095 0.065 0.085 0.132 0.022
2.8 0.143 0.074 0.076 0.085 0.118 0.031
thate distributing in roots to guarantee
roots growth and release inhibition of
ground parts.
Conclusions and Discus-
sions
The causes of different sensitive
degrees of crops vary to acidity. Some
might be caused by weak aluminum
tolerance and some is by pH ir-resis-
tance[13]. The research made by Xu[14]
proved that it is important to apply P,
K, and Mg fertilizers and micro ele-
ment fertilizers with lime in a rational
way. Application of lime in acidic soils
aims at removing Al3 + and H + which
are dangerous for crops, and then
supplying base ions. Many research-
es have been conducted on P fertil-
izer and lime, though the results are
different[15-19].
In the research, after lime applied
in red acidic soils, pH of soils was im-
proved in varying degrees and the test
indicated that suitable pH of a kind of
pasture crop would change after P fer-
tilizer applied. These demonstrated
that every pasture crop has an optimal
combination point of P fertilizer and
lime to promote growth of the plant,
which may not satisfy the growth of
plant height and tiller number based on
total biomass. When P fertilizer was
not applied, plant height, total tiller
number and biomass of the three
plants changed from increasing to de-
creasing as lime amount grew, and an
optimal combination point was ob-
tained. When P fertilizer was applied,
however, the three aspects kept simi-
lar trends, but the best combination
points of plant height and total
biomass changed little. Meanwhile, the
application of lime had insignificant ef-
fects on plant height, total tiller number
and biomass of Macroptilium atropur-
pureum and Chamaecrista rotundifo-
lia. What’s worse, dry matter of un-
derground part of Chamaecrista rotun-
difolia reduced with P fertilizer applied,
which suggested that Chamaecrista
rotundifolia enjoys high resistance to
acidity when P fertilizer is applied, fol-
lowed by Macroptilium atropurpureum.
With lime amount fixed, dry matter in
Macroptilium atropurpureum was max-
imal in the group with P fertilizer. On
the other hand, root shoot ratio of
Macroptilium atropurpureum in the
group with P fertilizers was higher
compared with the group without P,
assuming that lime amount is fixed.
Hence, it can be concluded that
Macroptilium atropurpureum requires
more P fertilizer to promote ground
parts and coordinate root shoot ratio. If
lime amount is the same, plant height,
total tiller number and dry matter of the
three plants were higher in the group
with P, compared with the group with-
out P, indicating that P is insufficient in
red soils.
Only two treatments were set in
the test, and it is necessary to add
more for further exploration. Still, the
research only showed the effects of P
fertilizer and lime on Trifolium repens,
Macroptilium atropurpureum and
Chamaecrista rotundifolia in natural
condition. Therefore, more attention
should be paid to effects of P fertilizer
and lime at harvesting pasture crops.
In addition, with lime amount the
same, application of P fertilizer would
enhance plant height, total tiller num-
ber and dry matter. When P fertilizer
was not applied, however, plant height
of the three plants achieved the peak
by lime at 1.4 g/kg which proves best
for improvement of acidity of red soils.
With P fertilizer at 200 mg/kg was ap-
plied, biomass of Trifolium repens and
Macroptilium atropurpureum achieved
the highest by lime at 2.1 g/kg, but to-
tal biomass of Chamaecrista rotundi-
folia was the highest by lime at 1.4 g/
kg. The research provides references
for planting and production of Trifolium
repens, Macroptilium atropurpureum
and Chamaecrista rotundifolia in
southern hilly regions.
References
[1] LEI GJ(雷宏军), ZHU DW(朱端卫), LIU
X(刘鑫), et al. Phosphorus adsorption-
desorption characteristics in acid soils
under amendment(酸性土壤在改良条件
下磷的吸附-解吸特性)[J]. Acta Pedo-
logica Sinica (土壤学报 ), 2004, 41(4):
636-640.
[2] WANG N(王宁), LI JY(李九玉), XU RK
(徐仁扣 ). Acidification of soil and im-
provement and management of acid soil
(土壤酸化及酸性土壤的改良和管理)[J].
Anhui Agricultural Science Bulletin(安徽
农学通报), 2007, 13(23): 48-51.
[3] WU G(吴刚), LI JY(李金英), ZENG XD
(曾晓舵 ). Bio-availability of calcium in
soil and the interaction with other ele-
ments(土壤钙的生物有效性及与其它元
素的相互作用 ) [J]. Soil and Environ-
mental Sciences(土壤与环境), 2002, 11
(3): 319-322.
[4] ZHAO MQ, CHEN X, SHI YF. Influence
of phosphorus fertilizer application on
available phosphorus and easy-loss
phosphorus content in red soil[J]. Agri-
cultural Science & Technology, 2012,
13(3): 570-573, 577.
[5] WANG YD(王跃东). Clover(三叶草)[M].
Kunming: Yunnan Science and Tech-
nology Press (昆明 : 云南科技出版社),
2000: 41-42.
[6] BAILEY JS, LAIDLAW AS. The interac-
tive effects of phosphorus, potassium,
lime and molybdenum on the growth
and morphology of white clover (Trifoli-
um repens L.)at establishment[J]. Grass
and Forage Science, 1999, 54: 1, 69-
76.
[7] LOWTHER WL. Interaction of lime and
seed pelleting on the nodulation and
growth of white clover. I. Glasshouse
trials. New Zealand[J]. Journal of Agri-
cultural Research, 1974, 17: 3, 317 -
325.
[8] LI BC(李邦成), XU ZH(徐志宏), YAN M
(闫敏), et al. Effect of lime application in
red soil on the growth and seed produc-
tion of white clover (施石灰对红壤地区
白三叶草生长发育及种子生产的影响)
[J]. Acta Agrectir Sinica (草地学报 ),
2006, 14(2): 193-195.
[9] ZHANG JQ(张久权), CHEN FX(陈福兴),
CAO WD(曹卫东). Introduction of Chama-
ecrista rotundifolia in red soil hilly region
and its application(圆叶决明在红壤丘陵
区的引种及应用研究)[J]. China Grass-
land(中国草地), 1994(4): 29-32.
[10] PENG LW (彭连武 ). On tropical and
subtropical excellent forage Macroptil-
643
Agricultural Science & Technology 2013
(Continued from page 639)
Responsible editor: Xiaoxue WANG Responsible proofreader: Xiaoyan WU
nology (农业科学与技术), 2010, 11(6):
183-185.
[7] LIU G (刘刚), YIN H (殷浩), TONG WH
(佟万红), et al. Effects of nitrogen, phos-
phorus and potassium on the DNJ yield
of mulberry leaf in spring(氮、磷、钾对春
季桑叶 1-脱氧野尻霉素含量和产量的
影响)[J]. Pratacultural Science (草业科
学), 2012(2): 274-279.
[8] WU ZY(吴志勇), DU ZH(杜之虎), XU H
(徐 鸿 ), et al. Principle and design of
“3414” fertilizer effect experiment
(“3414” 肥料效应试验原理与设计 )[J].
Xinjiang Farmland Reclamation Science
& Technology(新疆农垦科技), 2007(4):
63-65.
[9] LIU G(刘刚), YIN H(殷浩), LUO CY(罗
春燕), et al. Establishment of a mathe-
matical model for mulberry balanced fer-
tilization in hilly areas of Sichuan
Province(四川省丘陵地区桑树平衡施肥
的数学模型建立)[J]. Science of Sericul-
ture(蚕业科学 ), 2011, 37 (3): 0526 -
0531.
[10] YIN H, SHI X, SUN B, et al. Accumula-
tion of 1-deoxynojirimycin in silkworm,
Bombyx mori L.[J]. Journal of Zhejiang
University-Science, 2010, 11(4): 286-
291.
[11] SUN MY(孙敏耀), TANG WZ(唐文照),
LU X (卢霞), et al. Spectrophotometric
determination of total flavonoids in mul-
berry leaves of different harvest time
(分光光度法测定不同采收时间桑叶中
总 黄 酮 ) [J]. Chinese Traditional and
Herbal Drugs (中草药), 2004, 35(10):
1190-1191.
[12] LIU LL(刘利林), WANG S(王帅), YOU
YA (尤宜安 ). The Rudiment study on
nutritive value and active substance for
mulberry leaf (桑叶营养价值及部分活
性 物 质 的 初 步 研 究 ) [J]. Journal of
Tarim University (塔里木大学学报 ),
2010, 22(3): 25-28.
[13] CHEN JS (陈建生), XU PZ (徐培智),
TANG SH (唐拴虎 ), et al. Effects of
fertilization on cumulating characteris-
tics of dried matter mass of sweet corn
(施肥对甜玉米物质形成累积特征影响
研究 ) [J]. Plant Nutrition and Fertilizer
Science (植物营养与肥料学报), 2010,
16(1): 58-64.
[14] YIN H(殷浩), TONG WH(佟万红 ), YE
JJ (叶晶晶 ), et al. Effects of nitrogen
amount and nitrogen form on 1-de-
oxynojimycin content in mulberry leaf
(施氮量及氮素形态对桑叶中 1-脱氧野
尻霉素含量的影响)[J]. Journal of An-
hui Agricultural Sciences (安徽农业科
学), 2010, 38(32): 18129-18131.
[15] LU JW(鲁剑巍), CHEN F(陈防), LIAO
ZW (廖志文 ), et al. Effect of nitrogen
and potassium combined application
on mulberry (Morus atropurpurea
Roxb.) leaf yield and quality, and silk
cocoon quality(氮钾肥配合施用对桑叶
产量品质及蚕茧质量的影响)[J]. Plant
Nutrition and Fertilizer Science(植物营
养与肥料学报), 2007, 13(4): 719-724.
[16] ZENG Y(曾艳). Mulberry nutrition char-
acteristics and the effects of balanced
fertilization (桑树营养特性及平衡施肥
效应研究 ) [D]. Nanning: Guangxi Uni-
versity(南宁: 广西大学), 2007.
[17] WANG ZL(王泽林), CHEN JJ(陈继久).
The experiment on fertilization of NPK
ratio for the mulberry field for parent
egg production 1. the effect of different
NPK ratio of fertilization on spring
leaves proportion and quality(种茧育桑
园氮磷钾施肥最佳配方的试验 1. 春季
不同施肥比例对桑叶产、 质量的影响)
[J]. Bulletin of Sericulture (蚕桑通报 ),
2009, 40(4): 23-26.
!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!
Responsible editor: Xiaoxue WANG Responsible proofreader: Xiaoyan WU
ium atropurpureus (热带亚热带优良牧
草大翼豆的研究)[J]. China Grassland
(中国草地),1992(1): 52, 70.
[11] HUANG MF(黄梅芬 ), ZHAN X(占星 ),
KUI JX (奎嘉祥). Effect of phosphorus
and sulphur fertilizers on the yield of
siratro on torrid red soil(燥红土施用磷、
硫对斯伦春大翼豆产量的影响 ) [J].
Heilongjiang Animal Science and Vet-
erinary Medicine (黑龙江畜牧兽医 ),
2006(4): 47-49.
[12] BAO SD (鲍士旦). Soil and agricultural
chemistry analysis(土壤农化分析)[M].
The 3rd ed. Beijing: China Agriculture
Press(北京: 中国农业出版社), 2000.
[13] FU QL (傅庆林), MENG CF (孟赐福).
Effects of limestone application with
phosphate on growth and nutrient ab-
sorption of soybean on red soil(磷肥配
施石灰石粉对红壤上大豆生长和养分
吸收的影响)[J]. Soybean Science (大
豆科学), 1992, 11(2): 146-151.
[14] XU MG(徐明岗), ZHANG JQ(张久权),
WEN SL (文石林). Fertilizer effect and
fertilization of grass red soil hilly region
(南方红壤丘陵区牧草的肥料效应与施
肥 ) [J]. Pratacultural Science (草业科
学), 1997, 14(6): 21-23.
[15] HUANG MF(黄梅芬), WANG HX(王红
仙), CAO HY (曹后英), et al. Effects of
phosphorus, potassium and lime fertil-
izer on Vicia villosa yield(磷、钾肥和石
灰配施对光叶紫花苕产量的影响 )[J].
Forage and Feed (牧草与饲料), 2009,
3(2): 34-37, 52.
[16] HUANG JL (黄锦龙), LU FX (陆发熹).
Effects of phosphorus and quick lime
on latosolic red soil chemical proper-
ties and peanut growth (磷肥配施生石
灰对赤红壤化学性质及花生生长的影
响)[J]. Soil Fertilizers(土壤肥料), 1990
(3): 17-19.
[17] WEN SL(文石林), DONG CH(董春华),
RONG XM (荣湘民 ). The effects of
phosphate and lime application on
growth of Zea mexicana in red soils(磷
肥和石灰对红壤墨西哥玉米生长的影
响)[J]. Acta Agrectir Sinica(草地学报),
2010, 18(6): 870-874.
[18] DUGUMA B, MO ZX (莫治雄). Effects
of fertilizing phosphorus and quick lime
in acid soil on growth of Leucaena leu-
cocephala (在酸性土壤上施用石灰和
磷对银合欢生长的影响)[J]. Subtropi-
cal Plant Communication (亚热带植物
通讯), 1990(1): 57-58, 62.
[19] DUGUMA B, KANG BT, OKALI DUU.
Effect of liming and phosphorus appli-
cation on performance of leucaena leu-
cocaphala in acid soils [J]. Plant and
Soil, 1988, 110(1): 57-61.
Cambridge Scientific Abstracts (CSA)
In 2011, Agricultural Science & Technology’s full-texts have been included by Cambridge Scientific Abstracts (CSA). CSA is
a retrieval system published by Cambridge Information Group. With a history of more than 30 years, the company is an
important publisher of publishing abstracts and indexes with agent centers in Britain, France, Australia, Japan, Netherlands, New
Zealand, and Hong Kong China. The products include traditional printed abstracting and indexing journals, a variety of electronic
database which are retrieved through remote on-line and CD-ROM. Recently, the CSA launched network based (IDS) services,
which can retrieve hundreds of databases provided by CSA and CSA publishing partners.
For more information on CSA Full Text please sign in http://www.csa.com/
644