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作 者 ：马驿^1,2;陈杖榴¹

期 刊 ：应用生态学报 2009年 20卷 04期 页码：949-954

关键词：多样性；

摘 要 ：为了解恩诺沙星在环境中残留对土壤微生物的影响，应用扩增核糖体DNA限制性分析(ARDRA)对土壤细菌16S rDNA基因多样性进行了研究，并结合肠杆菌基因间的重复共有序列(ERIC-PCR)指纹图谱分析了恩诺沙星对土壤细菌种群基因多样性的影响.结果表明: 恩诺沙星作用于土壤后第35天，添加药物组的细菌总数均低于对照，且药物浓度越高，细菌数量越少;ARDRA分析将分离的土壤细菌分成了不同的操作分类单元(OTU)，各组的OTUs类型数分别为：Ⅰ组15个、Ⅱ组13个、Ⅲ组10个、Ⅳ组8个、Ⅴ组6个、Ⅵ组6个;对各组优势OTU进行了ERIC-PCR基因指纹图谱分析，Ⅰ～Ⅵ组的Shannon-Wiener指数分别为2.78、2.14、1.78、1.11、0.69和0.31，对照组的Margalef指数、Simpson指数和Pielou指数均明显高于添加药物组，且各多样性指数随药物浓度的增加而减少.

Abstract:In order to understand the effects of remained enrofloxacin (ENR) in e
nvironment on the diversity of soil microbial communities, amplified ribosomal D
NA restriction analysis (ARDRA) approach and genomic fingerprinting technique en
terobacterial repetitive intergenic consensus sequence-polymerase chain reactio
n (ERIC-PCR) were used to analyze the molecular diversity of 16S rDNA from soil
bacteria after ENR addition. The results showed that after the ENR addition for
35 days, the total count of soil bacteria was less than that of CK, and decreas
ed with increasing ENR concentration. The ARDRA divided the separated soil bacte
ria into different operational taxonomic units (OTU) groups, and the count of gr
oupⅠ to group Ⅵ was 15, 13, 10, 8, 6, and 6, respectively. Genomic fingerprint
ing analysis indicated that the Shannon-Wiener index of group Ⅰ to group Ⅵ wa
s 278, 214, 178, 111, 069 and 031, respectively, and the Margalef in
dex, Simpson index, and Pielou index of soil microbial community in CK were high
er than that in the soils in which ENR was added.

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