Abstract:A pot experiment with 3 levels of elemental sulfur (0, 30, and 60 mg S·kg-1) showed that sulfur fertilization on soybean increased the side roots number by 8.6%~33.2%, root dry weight by 6.6%~34.3%, root nodules number by 2.7% ~35.9% and dry weight by 13.0%~75.7%, chlorophyll content by 0.4~3.9 unit,and yield per plant by 7.3%~12.8%. Sulfur fertilization also increased the amount of soil bacteria, fungi and actinomycetes and the activities of peroxidase, urease, neutral phosphatase and polyphenoloxidase significantly. The effects of sulfur supply differed with its application rate, and 30 mg S·kg-1 was more appropriate for getting high soybean yield.