蚯蚓体腔细胞内的溶酶体能积累中性红染料.当受到环境胁迫时，溶酶体膜容易破裂，染料就逐步泄露到细胞质中，使细胞染成红色.中性红的保持时间(neutral red retention time，NRRT)可反映溶酶体受损情况.用不同浓度乙草胺和不同浓度Se处理赤子爱胜蚓(Eisenia fetida)，观察体腔细胞溶酶体中性红染色的保持时间.结果表明，随乙草胺和Se处理浓度的增高和处理时间的延长，NRRT缩短;NRRT与土壤中乙草胺残留的浓度呈明显的负相关，与蚯蚓体内富集的Se也有明显的负相关.可见，蚯蚓溶酶体中性红染色保持时间是测定土壤污染物的生物标志物，在环境风险评价和土壤监测中具有稳定性和准确性，可作为早期预警生物标志物.
The lysosome of Eisenia fetida can accumulate neutral red dye rapidly.When exposed to environment pollution,the membrane of the lysosome is easily to be cracked,and the dye shall leak to the cytoplasm gradually,making the cell red.Therefore,neutral red retention time (NRRT) can reflect the damaged degree of lysosome.In this paper,the NRRT of the lysosome in coelomocytes of E.fetida was determined after exposed to different concentrations of acetochlor and selenium.The results showed that with increasing exposure time and increasing concentration of acetochlor and Se,the NRRT was decreased significantly,and had significant correlations with the concentrations of acetochlor in soil and of accumulated Se in E.fetida’s body.NRRT could be served as a biomarker of soil pollution,and a promising tool for the early warning of potential danger.
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