Abstract:A corn-rape and a soybean-winter wheat rotation field in Guizhou Province were selected to measure N2O fluxes from sub-tropical upland soils.Based on the function of the DNDC model in well elucidating the seasonal changes of N2O flux and the effects of fertilising and tilling on them,it was thus used to assess the potential effect of variations in application of manure and N-fertilisers,fertilising timing,N-fertiliser types,fertilising depth,and tillage on N2O fluxes from sub-tropical upland soils.The results could be used to enact relevant policies,and to explore the potential for controlling N2O emissions from agricultural soils by changing fertilising and tilling.