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山乌桕Sapium discolor(Champ.ex Benth.)Muell.-Arg.



全 文 :·封面说明·
山乌桕 Sapium discolor (Champ. ex Benth. ) Muell.-Arg.
山乌桕为大戟科乌桕属植物,落叶乔木,高达 21 m,秋天
叶变为红色或黄红色,点缀着亚热带森林的美丽秋色。叶卵
状长椭圆形,长 4~10 cm,宽 2~5 cm,全缘,两面无毛,叶背淡
绿色;叶柄初为淡红色,于叶片基部有两枚膨大的腺体,叶柄
长 2~7 cm。花序长 4~10 cm,花淡黄色,雄花花梗丝状,长
0.1~0.3 cm,苞片卵形,两边各有一枚腺体,每苞片内有花 7
朵;小苞片长 0.1~0.12 cm,花萼杯状,具不整齐裂齿;雌花花
梗粗壮,长 0.5~0.9 cm,每苞片内有 1花,花萼裂片长 0.18~2
cm。花柱粗壮,柱头 3裂。蒴果,3室;种子近球形,薄被蜡质假
种皮。花期 4~6月,果成熟期 9~10月。
山乌桕主要分布在浙江、安徽、江西、福建、台湾、湖南、
湖北、广东、广西、云南、贵州、印度、越南等地,海拔 500~1 500 m,
较喜光,种子繁殖,是良好的园林观赏树种。
(刘仁林)
符合一般 RAPD-PCR的退火温度。通过实验获得优化的 20
μL反应体系和扩增程序能有效的进行柳叶蜡梅 RAPD的扩
增,为柳叶蜡梅的相关分子研究提供有益的参考。
参考文献:
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annua L[J].Molecular Breeding, 2002,9:251-257.
Optimization of RAPD Reaction System for
Chimonanthus salicfiolius
YANG Hua1, ZHANG Duhai1, CHEN Lei2, DU Guojian1
(1. Zhejiang Academy of Forestry, Hangzhou Zhejiang 310023, China;
2. Zhejiang University of Science and Technology, Hangzhou Zhejiang 310023, China)
Abstract: Taking Chimonanthus salicfiolius Hu as molecular study material, the building and optimization of its DNA
extraction method and PCR Amplification Reaction System were discussed. The results showed that the conditions being
suitable for RAPD- PCR as follows: 1×buffer, 20 ng template DNA, 2.75 mmol/L MgCl2, 0.25 mmol/L dNTPs, 2.0 U Taq
DNA polymerase, 0.4 μmol/L primer. The amplification cycling was 1 cycle at 94 ℃ for 2 min; 32 cycles at 94 ℃ for 60 s,
36 ℃ for 30 s, and 72 ℃ for 120 s, respectively; and 1 cycle at 72 ℃ for 10 min.
Key words: Chimonanthus salicfiolius Hu; RAPD; Optimization
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DOI:10.16259/j.cnki.36-1342/s.2010.05.004