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作 者 ：张建波, 金云峰, 王莎莎, 杨慧芹, 逄涛, 李军营, 崔明昆, 龚明

期 刊 ：生物技术通报 2016年 32卷 5期 页码：200-211

关键词：烟草；生长温度；淀粉代谢；荧光定量PCR；

Keywords:tobacco, growth temperature, starch metabolism, Real-time PCR,

摘 要 ：旨在明确不同温度对烟草不同生育期淀粉代谢的影响，以云烟87为材料，通过人工气候室模拟云南江川、贵州遵义和河南襄县的气温条件，在烟草移栽-团棵、团棵-现蕾和成熟期进行温度处理，研究了不同温度下不同时期烟草叶片中淀粉含量、酶活性的变化，并通过实时荧光定量PCR技术研究淀粉代谢相关基因表达的变化。结果表明，随烟株生长发育阶段的推进，淀粉含量不断增多，在成熟期含量达到最高。贵州遵义地区的温度条件有利于移栽-团棵期淀粉的积累和AGPase活性的升高；云南江川地区的温度条件有利于团棵-现蕾和成熟期淀粉含量的积累及AGPase、SSs活性的升高；河南襄县的温度条件淀粉积累较少。以上结果表明，温度通过影响淀粉代谢相关基因的表达，进而影响相关酶活性的升降和淀粉的代谢和运输，最终影响烟叶风格的形成。

Abstract:In order to clarify the effects of different temperatures on starch metabolism in different growth stages of tobacco，using Yunyan 87 as material growing in artificial climate chamber to simulate the different climates（Jiangchuan in Yunnan，Zunyi in Guizhou，and Xiang in Henan）in the stages of transplanting to resettling，resettling to squaring，and mature，we studied the effects of growth temperature on tobacco starch content and enzyme activity，and detected the expression changes of genes related to starch metabolism by RT-PCR. The results showed that，the starch content increased with the continuous growth of tobacco，and achieved the highest in the stage of mature. The temperature of Zunyi in Guizhou was beneficial to the accumulation of starch and the AGPase activity increased in the stage of transplanting to resettling. The temperature of Jiangchuan in Yunnan was conducive to the accumulation of starch content and the activities of AGPase and SSs raised in the stages of resettling to squaring and mature. The starch accumulation under the temperature of Xiang in Henan in any stage was little. The above results indicated that the temperature ultimately determined the formation of tobacco leave style by impacting the genes expressions of genes related to starch metabolism，thereby affecting the activities of related enzymes，as well as the metabolism and transport of starch.

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