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作 者 ：李志亮, 吴忠义, 杨清, 张希太, 叶嘉, 邢浩春, 陈建中, 黄丛林

期 刊 ：生物技术通报 2016年 32卷 5期 页码：61-68

关键词：玉米；愈伤组织；基因枪法；草丁膦；遗传转化；抗旱性；

Keywords:Zea mays, callus, microprojectile bombardment, glufosinate, genetic transformation, drought resistance,

摘 要 ：全球水资源短缺，干旱已是玉米生产发展的主要限制因素。通过培育抗旱能力提高的转P5CS基因玉米株系，为玉米抗旱遗传育种提供新材料。利用基因工程策略构建植物表达载体pBPC-P5CS-F129A，用基因枪法对玉米自交系京501幼胚愈伤组织进行遗传转化，在选择培养基中加入10 mg/L 的草丁膦进行筛选，对草丁膦抗性再生植株进行了PCR 检测和 Northern blot鉴定，对转P5CS基因玉米植株进行了抗旱性分析。结果表明，与对照相比，转基因植株的抗旱能力得到提高。干旱胁迫下，转P5CS基因玉米植株的脯氨酸含量比对照高，而丙二醛含量比对照低。

Abstract:A drought-resistance function gene，VaP5CS，was cloned from mothbean（Vigna aconitifolia）. Plant expression vector pBPC-P5CS-F129A was constructed using gene engineering strategy，then the genetic transformation of young embryo callus in maize inbred line Jing 501 was performed by microprojectile bombardment. Further，the transformed explants were directly selected on the medium containing 10 mg/L glufosinate，and the glufosinate-resistant plantlets were confirmed by PCR amplification and Northern blot identification. Finally，the different drought resistance indexes of P5CS-transgenic maize plants were analyzed. The results showed that the transgenic plants presented the improved drought resistance comparing to control plants. Under drought stress，the proline content in the P5CS-transgenic maize plant was higher than that in the control，while the MDA content was lower than that in the control.

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