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作 者 ：侯文胜 郭三堆 路明

期 刊 ：植物学通报 2003年 20卷 02期 页码：198-204

关键词：遗传转化；小麦；凝集素基因；质粒；蛋白质；雪花莲；

摘 要 ：利用适用于禾谷类作物的表达载体pGU4AGBar和pGBIU4AGBar，采用花粉管通道法， 将人工合成的雪花莲凝集素基因sgna导入了优良冬小麦品系西农2208和西农132，经PCR 和Southern blot鉴定，证明获得了20株导入了sgna基因的转基因植株，转化率约为0.2 8%～0.84%，并通过Western blot鉴定检测到了目的蛋白的表达。

Abstract:In the report, the gramineous expression vectors pGU4AGBar and pGBIU4AGBar were used, respectively. The sgna gene, a synthetical agglutinin gene of Galanthus navies, had been transferred into the winter wheat varieties Xinong 2208 and Xinong 132 by pollen-tube pathway. The PCR and Southern blotting analysis showed that 20 transgenic plants with sgna gene were obtained. Western blot ting analysis revealed that the target protein was expressed in the transgenic plants. The transformation frequency were 0.28%～0.84%.

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