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作 者 ：张景昱 张中林 苏宁 宋艳茹

期 刊 ：植物学通报 2001年 18卷 03期 页码：288-294

关键词：叶绿体转化；基因枪转化法；定点整合；

摘 要 ：叶绿体转化系统，独立于传统的核转化，为植物导入外源基因提供了新途径。它有如下优点：超量表达目的基因；以定点整合方式导入外源基因从而消除了位置效应及基因沉默；具原核表达方式，能以多顺反子的形式表达多个基因；母系遗传方式可防止基因扩散；基因产物区域化并能提供适于某些产物发挥功能的小环境等。随着这项技术在越来越多的领域发挥作用，其优越性逐渐得到认同。本文着重对此技术的特点、发展及应用作一综述。

Abstract:Chloroplast transformation, an alternative way to incorporate foreign genes into plant genome, has a number of advantages: higher transgene expression level; sitespecific introduction of foreign genes which leads to the absence of gene silencing and position effect; the ability to express polycistronic messages from a single promoter; uniparental inheritance that prevents pollen transmission of foreign DNA; provision of more favorable environments for cer tain biochemical reactions and for accumulating large amounts of gene products. It has become a useful tool in many fields. In this paper the characteristics, recent advances and application of chloroplast transformation are reviewed.

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