---

作 者 ：马庆虎 侯林林 吴欣 唐志强 宋艳茹

期 刊 ：植物学通报 1993年 10卷 01期 页码：43-45

关键词：黄瓜子叶扩张；BA；细胞分裂素；

摘 要 ：BA(benzyl adenine)专一性地促进离体黄瓜子叶的扩张。为了研究BA的作用机理，我们采用间接EIISA和HPLC的方法测定了子叶扩张过程中内源玉米素 (Z) 和玉米素核苷(ZR)含量的变化。离体黄瓜(Cucumis sativus,津研4号)子叶用100mg／1的BA培养，72小时之后，处理子叶鲜重的增加比对照高70％。Z+ZR在BA处理的子叶中有大量的积累。结果表明BA可能诱发了黄瓜子叶中的细胞分裂素生物合成和代谢的某些基因。

Abstract:Benzyladenine (BA) specifically promotes the expansion of excised cucumber cotyledons To investigate the mechanism of BA action, the fluctuation of endogenous zeatin (Z) and zeatin riboside (ZR) level was determined by indirect-ELISA and HPLC during the expansion process. The excised cucumber (Cucumis sativus, var Jin-ian No 4) cotyledons were incubated with 10 mg/1 of BA. Up to 72 hour, the fresh weight of the treated cotyledons increased more than 70% compared with that of control, large amount of Z+ZR were accumulated in the BA-treated cotyledons. This suggested that BA might trigger the cytokinin biosynthesis gene (s) in the cotyledons.

---