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作 者 ：耿德贵 韩燕 王义琴 李文彬 孙勇如

期 刊 ：植物学通报 2002年 19卷 03期 页码：290-295

关键词：杜氏盐藻；耐盐机制；基因工程；

摘 要 ：概述了杜氏盐藻（Dunaliella salina）的耐盐机制和基因工程的研究进展。盐藻的耐盐机制十分复杂，短时间内通过细胞体积的改变来调节渗透压平衡，之后通过甘油的合成与转化恢复细胞正常形态和大小。渗透调节过程中，还涉及到蛋白质的合成。cDNA文库和基因组文库已经建立；几种基因已被克隆，如碳酸酐酶基因和硝酸还原酶基因等；GUS（β_葡糖苷酸酶）基因已成功地转入盐藻细胞内。另外，对盐藻的基因工程作了简单的展望。

Abstract:Progress in studies on salt_resistant mechanism and gene engineering of Dunaliella salina is summed up. The salt_resistant mechanism is very complicated. It can maintain osmotic balance by rapid shrinking or swelling of cells, and then recover their original volume by glycerol synthesis or conversion. Some proteins are also synthesized after hyperosmotic shock. cDNA library and genomic DNA library of Dunliella salina have been constructed. Carbonic anhydrase gene, nitrate reductase gene and several other genes have been cloned. GUS(β_glucuronidase) gene has been successfully introduced in Dunliella salina. Prospects of gene engineering is also given.

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