The genetic transformation was achieved by pollen-mediated approach on maize ( Zea mays L.) inbred lines Tai 9101 and Zong 31. Plasmid DNA of pGLⅡ-RC-1 was mixed with fresh pollen of maize inbreds in sucrose solution. The pollens were treated by ultrasonication and collected, pollinated on silks of maize ears. Transformants were confirmed by dot blot hybridization, PCR amplification and PCR-Southern blot hybridization. The pollen-mediated transformation approach could circumvent the tedious tissue culture procedures like in particle bombardment and Agrobacterium infection, etc. This approach is simple, easy to operate, and could be widely used in practice.
花粉介导法获得玉米转基因植株
王景雪 孙毅* 崔贵梅 胡晶晶
（山西省农业生物技术研究中心，太原030031）
关键词： 玉米；花粉介导法；基因转化
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