Genetic structure of four populations in Castanopsis fargesii Franch. in Fujian Province was studied with microsatellite (SSR) markers. A high level of genetic variation was detected in the populations of C. fargesii by using SSR with A=9.0, Ne=4.8, He=0.65 and the population differentiation coefficient ( Fst ) was only 0.031. The distributions of alleles of all loci were significantly different among the populations of C. fargesii , and the population differentiation could be found according to the distributions of SSR alleles. Some rare alleles in the populations of C. fargesii were revealed by SSR: Fifteen of 54 alleles appeared in one or two populations with lower frequencies; conservation of these rare alleles is of great importance.
用SSR研究栲树群体遗传结构
徐立安* 李新军* 潘惠新 邹惠渝 尹佟明 黄敏仁*
（南京林业大学林木遗传和基因工程国家林业局重点实验室，南京210037）
摘要：利用微卫星（SSR）分子标记对福建省内4个栲树（Castanopsis fargesiiFranch.）群体遗传结构进行了研究。SSR标记揭示了栲树群体丰富的遗传变异：平均等位基因数A=9.0，平均有效等位基因数N e=4.8，平均期望杂合度He=0.65，而群体具有较低的Fst值（F st=0.031）。SSR每个位点的等位基因频率分布在栲树群体间都存在显著或极显著差异，表明根据SSR等位基因频率分布亦能了解群体的分化。SSR标记使栲树群体中一些稀有等位基因得以表现，54个SSR等位基因中有15个等位基因仅出现在1个或2个群体中，且频率较低，在遗传多样性保护中更应注重保护这些稀有的等位变异。
关键词： 微卫星；栲树；群体遗传结构；等位基因频率
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