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作 者 ：肖 炜^1,2, 王峥峰¹, 曹洪麟¹, 叶万辉^1*, 黄国敏^1,2

期 刊 ：广西植物 2010年 1期 页码：70-74

关键词：鼎湖血桐；点格局分析；径级结构分析；微卫星；r-对策；阳生植物；

Keywords:Macaranga sampsonii, Points pattern analysis, DBH-class structure analysis, microsatellite, r-strategy, light-dependent plant,

摘 要 ：采用点格局分析、径级结构分析以及微卫星筛选等方法,对鼎湖山保护区20 hm²永久样地中的鼎湖血桐进行了种群生物学及遗传多样性研究。结果表明:鼎湖血桐是一种r-对策型阳生植物,种群年龄结构呈金字塔型; 植株受光照不足的胁迫,种群大部分个体聚集分布于光照充足的林窗中; 通过磁珠富集法筛选出鼎湖血桐特异性微卫星引物13对,其中2对具备多态性,多态百分率为15.4%,多态信息含量在0～0.3734之间,平均期望杂合度为0.0756,远低于同属其它植物。研究表明鼎湖血桐对环境变化的适应范围较窄,遗传多样性较低,应对其种质资源进行多样性评估与保护。

Abstract:An analysis of population biology and genetic diversity of Macaranga sampsonii was taken in DHS-20-hm² permanent plots,which locates on the Dinghushan natural reserve. By use of point pattern analysis and DBH-class structure analysis,it was concluded that M.sampsonii was a light-dependent egy,whose population age structure was approximately pyramid-like. The population habitat was restricted in the canopy gaps because of sunlight deficiency stress. Thirteen microsatellite loci were isolated by methods of magnetic beads enrichment,in which only two loci(15.4%)were polymorphic. The polymorphic information content of these loci ranged from 0 to 0.3734.The average expected heterozygosity of M.sampsonii was 0.0756,which was obviously lower than other Macaranga species. These results indicated that M.sampsonii was in a narrow environment adaptation range and a low population genetic diversity. We should take measures to evaluate and protect its germplasm resource.

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