The oxygen evolution, thermal dissipation, and photochemical energy storage of three hybrid poplar clones, namely the triploid clone B342, the diploid clone B11［( Populus alba×P. glandulosa)×(P.tomentosa×P.bolleana)］ , and the triploid clone B346 ［ (P.tomentosa×P. bolleana)×(P. alba×P.glandulosa )］, under light stress were studied using photoacoustics. The oxygen evolution signal and photochemical energy storage varied negatively with the pretreatment-PFD (photon flux density), whereas the thermal signal varied positively with the pretreatment-PFD. Photochemical energy storage was reallocated to PSⅡ more than to PSⅠ, while the photochemical energy storage in PSⅠ was more stable than that in PSⅡ when subjected to light stress. The inhibitors streptomycin (SM), dithiothreitol (DTT) and sodium fluoride (NaF) could all affect the oxygen evolution signal. Clones B11 and B342 were more resistant to light stress than clone B346.
光胁迫对杂种杨无性系光合放氧、光化学能量贮藏的影响
张守仁1,2 高荣孚2
（1. 中国科学院植物研究所植被数量生态学开放研究实验室，北京100093；2. 北京林业大学生物学院，北京100083）
摘要：以光声光谱技术对经不同光强预处理的 3个杂种杨无性系即三倍体无性系B34 2、二倍体无性系B1 1[(Populusalba×P .glandulosa)× (P .tomentosa×P .bolleana) ]和三倍体无性系B34 6 [(P .tomentosa×P .bolleana)×(P .alba×P .glandulosa) ]的光合放氧、光声热信号及光化学能量贮藏进行测定。叶圆片的光声放氧信号和光化学能量贮藏与预处理光强度成反比 ;而光声热信号与预处理光强度成正比。光化学能量贮藏分配到PSⅡ的份额要比分配到PSⅠ的多。在强光胁迫下PSⅠ的光化学能量贮藏要比PSⅡ的光化学能量贮藏稳定。 3种抑制剂 (strepto mycin ,dithiothreitol,sodiumfluoride)均对叶圆片的光声放氧信号有影响。无性系B1 1和B34 2抵抗光胁迫的能力比无性系B34 6高
关键词： 杂种杨无性系；光胁迫；光声放氧信号；光化学能量贮藏；光声光谱技术；抑制剂
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