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Gene Expression Analysis of a Proline-rich Protein from Bean Under Biotic and Abiotic Stress


The PvSR1 ( Phaseolus vulgaris stress-related protein) gene encoding an 11 kD proline-rich protein (PRP) was constitutively expressed in the primary leaf tissue of bean ( Phaseolus vulgaris L. cv. Saxa). Time course studies of mRNA accumulation of the PvSR1 has been studied in this plant against virus infection and various abiotic stresses. Northern blot analysis revealed that the gene expression of PvSR1 was greatly enhanced in the leaf tissue by A1MV (alfalfa mosaic virus) infection, wounding, heat shock, tN, drought and salt stress. The PvSR1 transcripts could also be increased by the exogenous inducing factors such as salicylic acid and 11202. The precise biological roles of PvSR1 is unknown, but the expression of PvSR1 genes in various form of stress suggest that PvSR1 may play an important role during the stress, in maintaining cellular integrity by forming strong hnkages with the cell wall.


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