Abstract:The hybrids from different generations of autotetraploid rice (Oryza sativa L. ) and the original autotetraploid rice (indica and japonica) were used for anther culture, and the pollen-plantlets from them were induced. Due to the significant difference on phenotype among the trisomics and between trisomics and diploid, 15 lines of 4390 H1 induced plants were selected for chromosome study. Their PMC meiosis were observed. The results showed that the chromosomes from these plants consisted of 2n, 4n and aneuploids, and their ratios were 88.00%, 5.53% and 6.47% respectively. 272 trisomics from 284 aneuploids were identified, which acounted for 6.20% of all the pollen-plants. According to the special characters from the whole set of trisomics, they were classified as 9 types. The 9124- 7 trisomics were designated as triplo-8 by the pachytene analysis. Sowing the seeds of triplo8, the transmission rate of extra chromosome was calculated at the seedling stage of H2. The rate of trisomic was 34.11% of all plants, the agronomic characters were similar to the H1 parent plants.