Abstract:Samples of 8 populations representing Quercus aquifolioides Rehd. et Wils. were collected. Isozymic electrophoresis was used to analyze the genetic variability and it was found that the level of genetic variability varied regularly with different altitudes: the level of genetic variability between the altitudes of 2 400 to 3 300 metres was high, and it became low at the altitudes of 3 800 and 2 200 metres. It was also found that the factors, such as human interference and sunlight, had strong effects on the level of genetic variability.