Abstract:An antagonistic strain, Bacillus spp. TG26 isolated from roots of a towel gourd (Luffa cylindrica (L.) Roem.), secretes large amount of antifungal proteins with strong inhibiting activity against Gibberella zeae, Fusarium oxysporum f. vasinfectum and niveum, ,4hernarict longipes, Trichoderma viride as well as bacterial pathogens of Xanthomonas campestris pv. oryzae and Psendomonas solanaceanum. Crude proteins obtained by precipitation of the cellfree culture of TG26 strain with ammonium sulphate at 70% saturation are over 130 mg/L. The optimal culture conditions of TG26 strain are as following: BPY medium, initial pH 7.0, temperature 30 ℃ and cultivation time 36 hours.