Abstract:Fusarium wilt of banana, a classic soilborne disease, is considered as one of the most serious threats to banana. The objectives of this study were to supply newly available strain from rhizosphere of healthy banana and effectively against the responsible pathogen Fusarium oxysporum f.sp.cubense (FOC). The result showed that a total of 74 bacteria strains were isolated. Among these strains, C-4 significantly inhibited mycelial growth of FOC with the relative inhibited efficiency above 80%. Isolate C-4 showed a wide range of antagonism against some pathogens in vitro test, such as Fusarium oxysporum f.sp. lycopersici, Curvularia lunata, Colletotrichum gloeosporioides, Fusarium oxysporium f.sp. vasinfectum, Verticillium dahliae, Gibberella zeae, Fusarium oxysporum f.sp. niveum. From the results of Biolog analysis and 16S rDNA sequence, isolate C-4 was identified as Bacillus licheniformis.Isolate C-4 cultured at media of YPGA or PDA, pH 7.8-8.0, temperature 32-35℃, cultural time 40-52h, growth and inhibition was optimal. Pot trials showed that strains C-4 could effectively control banana wilt and significantly reduced the disease severity when applied as a root dip treatment 30 days later.