Abstract:Five crystalline compounds A, B, D, E, F were isolated for the first time from the roots of Mallotus apelta were identified as erythrodiol-3-acetate for A, β-sitosterol for B, 3β, 29-dihydroxylupane for D, ursolic acid acetate for E, except for F, via chemical and spectroscopic analysis. It was shown that the crystallines of A, B, D, E, and F possessed some bacteriostatic activities on Staphylococus aureus, Bacillus subtilis, Escherichia coli and Bacillus pyocyaneum.