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期 刊 ：中国中药杂志 2007年 32卷 10期 页码：932.

关键词：白背叶；化学成分；HPLC；葫芦巴苷Ⅱ；

Keywords:Mallotus apelta, chemical constituent, HPLC, vicenin Ⅱ,

摘 要 ：目的：研究白背叶的化学成分并建立含量测定方法。方法：聚酰胺柱色谱,Sephadex LH-20柱色谱,制备液相色谱分离纯化,理化性质和光谱数据进行结构鉴定；高效液相色谱条件,Inertsil ODS-3 C₁₈(4.6 mm×150 mm,5μm)色谱柱,流动相为甲醇-水(24∶76),检测波长335 nm。结果：从白背叶正丁醇萃取部位分离得到一化合物,鉴定为葫芦巴苷Ⅱ；葫芦巴苷Ⅱ的线性范围为0.053～10.60 μg,r=0.999 9；平均回收率为99.32%,RSD 1.82%(n=6)。结论：该化合物系首次从该植物中分离得到,并首次利用高效液相色谱法建立了测定白背叶中葫芦巴苷Ⅱ含量的方法,方法简便、准确可靠。

Abstract:Objective: To investigate the chemical constituents and establish a quantitative method of Mallotus apelta. Method: Compound was isolated by silica gel, Sephadex LH-20 column chromatography and Pre-HPLC chromatography. Its structure was identified by physicochemical properties and spectral evidences. The content of M. apelta was determined by HPLC. Chromatographic conditions included Inertsil ODS-3 C₁₈ column (4.6 mm×150 mm, 5 μm) and the mobile phase consisting of a mixture of methanol-water (24∶76). The detection wavelength was set at 335 nm. Result: One compound was isolated from n-butanol extract of the M. apelta and its structure was identified as vicenin Ⅱ. The calibration cure was linear in the range of 0.053-10.60 μg (r=0.999 9), the average recovery was 99.32%, RSD 1.82% (n=6). Conclusion: The compound was isolated from this plant for the first time. The method to determine the content of vicenin Ⅱ by HPLC was established for the first time. This method is simple, accurate and reliable.

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