Abstract:A comparative study of middle layer both in sterile and fertile anthers of Tai Gu wheat was made on electron-microscopic level. The submicroscopic structure of middle layer in fertile form was found that its middle layer degeneration was somewhat later than the period mentioned in literatures, and lasted somewhat as long as that of tapetal layer. On the other hand, there was a tendency of early disintegration of middle layer in sterile anthers. When abnormal meiosis of pollen mother cells was progressing and followed by pollen mother cells abortion one after another, the middle layers degenerated gradually, and appeared as irregular dark regions or empty spaces.