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A Comparative Study on Function and Membrane Proteins of Chloroplasts of Three Species of Cephalotaxus in Late Winter


Three species of evergreen Cephalotaxus (C. fortunei Hook. f., C. sinensis Li and
C. harringtonia cv. Fastigiata) were used as materials to study some functional   properties of
chloroplasts.  It is found that the oxygenevolving capacity of the chloroplasts from these plants
is inhibited but partial reaction of PS-II and effect of Mg2+ on energy distribution between
two photosystems are detectable during the winter. Seasonal effects on the functional properties
of chloroplasts from evergreen Cephalotaxus are similar to that of conifer chloroplasts.
      The ratio between F685, F695 and F735 of fluorescence emission spectra at 77°K of ch-
loroplasts is different among these three species. It is found by using SDS-PAGE that the num-
ber of polypeptide resolved from thylakoid membrane of C. harringtonia cv. Fastigiata sub-
stantially differs from that of C. fortunei Hook.f. and C. sinensis Li. The result shows that the
fluorescence emission spectrum feature and polypeptide composition of thylakoid membrane may
be used as a tool for systematics of the genus Cephalotaxus.


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